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Barbie Doll Analysis Essays and for european exploration Research Papers. Element! Barbie dolls were born in the Wisconsin state of American 50 years ago. Motives Exploration! They are no longer simple dolls , but a . symbol of American women, American culture and an ideal embodiment of global female. The following essay will firstly discuss Barbie dolls ’ historical and cultural background of the atkinson, era when they appeared. This part includes three aspects, which are the for european exploration, consumer society of America at that time, the prevailing of hedonism in the consumer society and is zone of proximal the enhancement of self-awareness among. Barbie , Mattel , Ruth Handler 1959 Words | 6 Pages. The Essay Analysis of Barbie Doll. images of how we think life is supposed to be. Although, who’s to say what’s right and what’s wrong with the way we interpret things?

Marge Piercy, who . wrote the poem “ Barbie Doll ”, has a very strong view of how destructive social pressure can be to a girl through her transitioning stages into exploration a woman. She expresses how the Barbie doll , the toy figurine that woman idealize, is, in fact, a method of Essay NOT Support corruption to a young girl. First and for most we must understand who the persona is in the poem, which. Barbie , Boleslaw Prus , Fashion doll 1296 Words | 4 Pages. Motives Exploration! Barbie Doll The poem “ Barbie Doll ” wrote by atkinson and shiffrin, Marge Piercy describes four different stanzas about the . cultural and societal expectations that American people place on young girls. The poem describes a short summary over the life of a young female from birth to motives exploration death.

The start of the poem is Essay Most and Brazen Dream, written with a happy tone emphasizing about the Barbie doll and its characteristics. Depression and motives for european exploration sadness then takes place starting with the very negative line “You have a great big nose and what mean fat legs.” and then. Female , Girl , Woman 1582 Words | 4 Pages. For European! Executive Summary Barbie has become one of the best selling toy brands in what is zone of proximal the world helping Mattel become one of the largest toy makers. Motives For European Exploration! . Mattel has had much success with it popular Barbie dolls for over fifty years. However it seems every product has a life cycle and in recent years Barbie has begun to decline in popularity due to and shiffrin memory model recent technological trends and stiff competition. Today young girls have become more interested in mp3 players and ipods rather than playing with dolls . At home and abroad.

Barbie , Bratz , Fashion doll 1639 Words | 6 Pages. The Barbie Doll : Perfect in an Imperfect World Imagine long blonde hair, perfect tan skin, a tall slender body with perfect . measurements and exploration outfits that only accentuate the perfect features. It might be hard, or impossible, to bring to mind a human being who could fit these characteristics. Essay Dream Writing! If I were to say, long blonde hair, perfect tan skin, a tall, slender body with perfect measurements and for european outfits that only accentuate her plastic body, what comes to mind now? Young children have received. Auburn hair , Barbie , Body image 874 Words | 3 Pages. Barbie Topic: Barbie General Function: To inform . Specific purpose: To inform my audience of the history of the Barbie Doll . Essay On A Most Bold And Brazen To Pursue Writing! Organization Design: Topical Introduction Attention-Arousing and Orienting Material: When you think long hair, perfect skin, trendy clothes, and a rockin’ body, what comes to every little girls mind?

Barbie of course! Credibility: As a child I had. Barbie , Bild Lilli doll , Fashion doll 926 Words | 5 Pages. The Barbie Doll Effect Society's idea to be attractive is to be nothing less than ideal. To lack perfection is not acceptable . in society. Motives For European Exploration! Also society tells people how to dress and act, having people be and much mean look a certain way to be accepted. Motives For European Exploration! The desire to be accepted can destroy ones’ self-esteem and many lose sight of their own true beauty. Many will do whatever it takes to not be, say, or do what society thinks is Essay on Christians Should the Death Penalty, disturbing.

Marge Piercy’s poem “ Barbie Doll ,” written in 1973, is a powerful. Fashion doll , Female , Girl 997 Words | 3 Pages. Barbie Doll by Marge Piercy Analysis. Not Everyone is Made with a Barbie Doll Mold “ Barbie Doll ” by Marge Piercy is about a girl who is a . normal child growing up; playing with dolls , miniature kitchen items and pretend make-up. It quickly takes an interesting turn when a pubescent child makes fun of her nose and legs and she was advised to exercise and diet despite the fact that she was intelligent and healthy. The poem continues on by the girl cutting her legs and nose and for european a bizarre visual of her laying in a casket with an ending. ARIA Charts , Barbie , Fashion doll 1404 Words | 4 Pages. Margie Pearcy's Barbie Doll Margie Pearcy's Barbie Doll details the image that society . projects upon Should the Death Penalty and expects from its young female population. From an early age these young women struggle to conform to the standards that society has defined for them. Motives! The results often are disastrous, leading to emotional conflicts that are often difficult if not impossible to resolve. Beautiful, flawless dolls such as Barbie are frequently the first source of association that little girls have with.

Barbie , Boy , Childhood 887 Words | 3 Pages. What Is Zone Of Proximal! ?Emily Choi Professor Kevin Cavanaugh ENG 102 April/ 9th / 2014 “ Barbie Doll ” reader Response Barbie . Exploration! Doll by and shiffrin model, Marge Piercy is a poem that highlights the social roles of the exploration, females in comparison to a doll . The way society wants women to look is memory, like the title of the for european exploration, poem, “ Barbie Doll ”. Society continuously forces women to fit into a certain standard of write how society defines beautiful. In the poem, the author delivers the message of feminism to the reader by telling a story of a child who is motives for european, a girl. Fashion doll , Female , Gender role 771 Words | 3 Pages. Barbie Doll: Is Barbie The Ideal Woman? Is Barbie the is zone, ideal woman? For generations she’s been the doll that little girls have aspired to be–a party girl, career woman . and bathing beauty all wrapped into one . In Marge Piercy’s poem entitled Barbie Doll , the title underscores the motives, theme of the poem, which is that girls are ultimately and fatally entrapped by society’s narrow definitions of development feminine behavior and for european exploration beauty. By comparing the young lady in the poem to a Barbie doll , the author reveals the irony of the atkinson and shiffrin memory, title.

In the poem, the. Barbie , Boy , Dolls 2312 Words | 6 Pages. Barbie Dolls Barbie has been a BIG deal in the toy aisle. She's also morphed through a variety of careers, gained . and lost friends, and for european acquired an nothing amazing number of possessions. Motives For European! Barbie was the suggestion of Ruth Handler, wife of of proximal one of Mattel's co-founders, Elliot. For European Exploration! Handler had noticed that her own daughter enjoyed playing with adult dolls as much as she did baby dolls . At that time, though, most adult dolls were paper or cardboard. Handler suggested an adult fashion doll to her husband. What Does Ado About! The all-male.

Barbie 1526 Words | 5 Pages. the last summer of her “childhood” playing with Barbie dolls with her little sister, Jennie. ?I think little girls playing with . Barbie dolls are trying to grow up, because Barbie is a young adult and has a fully developed body, wears makeup, and drives. She also engages in many different careers, making her a role model to young girls. I think Nichole playing with Barbie dolls symbolizes her growing up, as ironic as that is. Barbie’s Debut ? Barbie was introduced in motives for european exploration 1959 at the American Toy Fair. Barbie , Bild Lilli doll , Doll 405 Words | 11 Pages. Essay On Christians Should NOT Support Penalty! Barbie : The American Girl Goes Global Barbie which is produced by Mattle is the most known and successful baby . doll all around the world.

So there is a reason underlying this success. For European! Firstly, Mattel’s global marketing strategy involves adapting fashion trends and cultural differences to its product. Since, Barbie has been successfully adapted to write introduction for assignment cultural differences in the United States, Mattel tried to adopt a regiocentric approach for Western European market. However, Mattel improved a. For European! Barbie , Competition , Culture 1091 Words | 3 Pages. Variety is the spice of the Barbie Doll Marketing Success. Essay! First introduced to the US market in for european 1959, Barbie has . grown into of poetry a global cultural phenomena. From just one female doll , the Barbie family has grown and includes over 38 friends and motives for european relatives. There are now dolls that represent multiple ethnicities, males, different age groups, different abilities, different lifestyles, etc.

All totaled, there are now over one hundred and fifty different dolls under the Barbie brand. Quality Quality has. Barbie , Bild Lilli doll , Doll 1049 Words | 3 Pages. Daus-Haberle English 101 12 December 2014 The Barbie Doll Effect Perhaps one of the how to write, most famous toys in American history, the . Barbie doll , is motives for european exploration, a staple in what of proximal development the toy chest of little girls everywhere. For European! Barbie dolls have been the ideal role model for girls for many years. Barbie is pretty, has the is zone of proximal, perfect body, has the perfect boyfriend and family, and has every accessory in the world; she is for european, perfectly perfect. Memory Model! The average girl, from the ages 3-11, owns at exploration least ten Barbie dolls and spends hours playing with them.

Barbie , Bild Lilli doll , Bratz 1727 Words | 6 Pages. Memory! Nicole Goodman: Barbie Doll Poetic Analysis. Nicole Goodman Enc1141 Monday- Wednesday 2:00 – 3:15 September 24, 2012 Poetic analysis Barbie Doll poetic . For European! analysis The poem “ Barbie Doll ” by and shiffrin, Margie Piercy is about a girl who, at a very young age, is teased about her looks. She lives her whole life based upon what one fellow child says to her one day until she can not take seeing herself as how he saw her anymore. The poem shows us that even one small joke about for european exploration someone can affect her forever.

The poem opens with the first stanza describing. ARIA Charts , Childhood , Debut albums 1040 Words | 3 Pages. “ Barbie Doll ” by Marge Piercy The poem “ Barbie Doll ’ by Marge Piercy dramatizes the Essay Bold and Brazen Dream, conflict between . stereotyping and perfection within society. Exploration! The title accentuates the theme of the what does nothing mean, poem; the Doll symbolizes society’s interpretation of beauty and motives exploration a reflection of how the girl’s ideas of this beauty shape her self-worth. According to how to Steven Ratiner, author of motives for european exploration Giving Their World: Conversations with Contemporary Poets, it is Piercy’s realistic interpretations of life experience which develops the. Barbie , Boleslaw Prus , Dolls 1709 Words | 4 Pages. Atkinson Model! What toy has everything anyone could ever ask for? (Pause for for european exploration, 2 seconds) If you are thinking of Barbie , then you are correct. . Barbie was my favorite toy as a child and it still is for many little girls today. Essay On Christians! Today, I am going to talk about how Barbie was designed, how she has changed over the years, and how Barbie affects American culture. Here is exploration, Barbie’s story.

In 1930, a young girl fell in how to introduction love with a boy. The girl had high hopes of going off to college to become a success woman with a. Barbie , Bild Lilli doll , Fashion doll 1061 Words | 3 Pages. Barbie: Should The Doll Be Modified? Barbie : Should the Doll be Modified?

Jordan Sidebottom ENGL 1302 Professor . Exploration! Baggaley February 3, 2014 Sidebottom 1 Jordan Sidebottom ENGL 1302 Professor Baggaley February 3, 2014 Barbie : Should the on Christians Should NOT Support, Doll be Modified? Since Barbie was first launched by Mattel in 1959 over 10 million have been sold. “Somewhere in for european the world, a Barbie is sold ever half-second”(Dittmar, Halliwell, and Ive). Out of all Mattel profits, Barbie makes. Barbie , Body image , Bratz 1386 Words | 6 Pages. Poetry Analysis Essay As women, we are born into this world with high expectations. There is an image of perfection that our parents could . only hope we fulfill; but as parents love us no matter what our physical outcome may be, society, on the other hand, does not so understand. Barbie Doll by Marge Piercy is a painfully honest eye-opening poem dwelling on the severity of an how to average girl feeling as if she is not beautiful enough. The crucial elements of poetry include imagery, similes, symbolism. Barbie , Big Nose Kate , Boleslaw Prus 1298 Words | 4 Pages. popular Barbie doll created by Mattel, the idealized image of a woman in our patriarchal society is motives for european exploration, one who takes care of the . home and model is flawlessly beautiful with perfect skin, long legs, small waist, and slender figure.

The Barbie doll is used as a tool for motives exploration, patriarchy in Essay Should NOT Support the Death Penalty that it reinforces the motives for european exploration, notion that women should be domestic workers and maintain a feminine outer appearance. Also, patriarchal values affect girls starting at a young age as they unconsciously begin to on Christians Should believe that Barbie is what. Motives Exploration! Feminism , Gender , Gender role 2455 Words | 6 Pages. On A Bold To Pursue Writing! A Young Woman's Suicide Barbie Doll by Marge Piercy and Suicide Note written by Janice Mirikitani are both poems about motives for european exploration . the story of a young woman's reasons for committing suicide. Both explain that their suicides were influenced by their feelings of not being 'perfect', rather or that the people around them did not think they were 'perfect' or 'good enough'. Though the tone, language, as well as the story written in the poems are different they both share the how to write introduction for assignment, theme of a young woman's conflict.

Death , Suicide , Suicide note 932 Words | 3 Pages. BARBIE DOLL by MARGE PIERCY NUR AIN BINTI MOHAMMAD HAMSANI (AP00141) LITAPP AP201 In my understanding basically the poet is . Motives For European Exploration! describing the truth within the society about the idea of beautiful or perfect women. The poem can be said as a story of a girl from the very beginning to the end. There are four stanzas for how to, each stage of her life; a child, a teenager, an adult and after she is dead. Motives! Each of them describes how the girl confronts all the hardships and difficulties in her life in order to be. Aesthetics , Boleslaw Prus , Fashion doll 1292 Words | 4 Pages. Barbie Doll ” by Marge Piercy 1. In approaching the what much nothing, assignment question, I took as a starting point from an unknown guest . comment:” From the moment she is motives, born, this girl is exposed to the expectations of society and goes through life encountering the harsh realities of element not meeting those expectations. The need to live up to the world’s idea of motives for european exploration beauty fuels years of severe self scrutiny that tears down any and all of her self confidence- preventing her from loving any part of herself. The constant.

Barbie , Fashion doll , Sociology 1015 Words | 3 Pages. Mattel Inc. is the does much mean, induction industry of the Barbie dolls . The first Barbie doll was invented in 1959 . and since then, the Barbie dolls have been around for decades and have become some of the most popular dolls in motives exploration the world. Memory! Young girls often enjoy playing make believe with their Barbie dolls through the way they dress, and make the exploration, dolls behave during play time. Most young girls see themselves through their dolls and Essay NOT Support Penalty so the young children view the Barbie dolls as their idol and future self when they. Barbie , Barbie's careers , Doll 1234 Words | 3 Pages. Barbie Doll Revolutionized Toy Industry.

Barbie Doll Revolutionized Toy Industry By Belem Ramos From left, Cherity and for european Amanda Pierce display their . Barbie collection. Photo by Sandra Pierce. Flashy clothes, the is zone of proximal, perfect boyfriend, a Corvette, Ferrari, full size apartment with beautiful furniture and a boat. She's the woman who has everything and every year receives more. Since her introduction in 1959, the Barbie doll may be the most influential icon of American culture in the late twentieth century.

Barbie's success may be attributed. Barbie , Bild Lilli doll , Doll 1338 Words | 5 Pages. Motives For European! Barbie Doll: Society's Whims Are Not To Be Fulfilled. Society’s Whims Are NOT To Be Fulfilled In the poem “ Barbie Doll ,” author Marge Piercy utilizes four well-developed stanzas . to depict a scornful view of American society. Of Proximal! Applicable to all time periods, “ Barbie Doll ” narrates the motives for european, short-lived life of a young girl despised by society for how to for assignment, her appearance. Barbie Doll is like a fairytale, full of plasticity, fakeness, and fantasy.

However, unlike a fairytale, “ Barbie Doll ” ends with society applauding the funeral of motives for european exploration a princess that was torn apart. Barbie , Big Nose Kate , Fashion doll 901 Words | 3 Pages. Informative Speech about Barbie Dolls. Objective: I will give an does ado about informative speech about the for european exploration, history of Mattell’s Barbie Doll . How To Introduction For Assignment! I. Introduction A. Attention . Getter: a. Girls, this is a question for motives exploration, you. Element Of Poetry! How many of motives you have had Barbie dolls growing up? (Have time to answer) b. Well, believe it or not, our beloved Barbie dolls have a whole lot of history behind them.

B. Tie to the audience: a. I think we, as girls, can all vouch that Barbie dolls were all the rage and so much fun when we were younger, and of poetry even when our grandmother. Barbie , Bild Lilli doll , Doll 720 Words | 3 Pages. Motives For European! Pressure of Society in “Barbie Doll” woman looks, acts, and behaves like. Whenever a young woman fails to live up to these outrageous ideas they are belittled and told to of poetry change what they look . like and how they behave. Motives Exploration! This is exactly what happens to the girl in Essay Most and Brazen Dream Marge Piercy’s poem “ Barbie Doll ” (236). The pressure that society was putting on the shoulders of this girl became too much one day. She finally decided to give up on being herself and motives exploration become who the and shiffrin memory, world wanted her to be. The end of the poem seems to exploration be speaking of Essay NOT Support her suicidal. Motives For European Exploration! Barbie , Boleslaw Prus , Death 1144 Words | 3 Pages. Barbie Doll: Societies Destructive Idealism. Societies Destructive Idealism Marge Piercy wrote the poem “ Barbie Doll ” in her 1973 collection, To Be Of Use.

The story . Nothing! follows the life of a young girl growing up with modern expectations that she struggles to conform to. Barbie Doll uses different aspects of a woman’s life to express the different pressures on motives exploration, women in today’s society. What Is Zone! The first characteristic Piercy uses to exploration emphasize the stereotypes attached to women are images, colors, and toys that are traditionally associated with. Barbie , Boleslaw Prus , Fashion doll 1403 Words | 4 Pages. BARBIE HISTORY Let me begin by telling a story. A young girl meets a boy and they fall in love.

The girl graduates from high school. She . had visions of a great career success in the real world. She wanted to go to college anyways because her parents thought traditional. The girl went to college and married the boy too. They raised two children, Barbie and Ken. They are the creators of the company Mattel and the makers of Barbie , Ruth and Elliot Handler. The reason of me telling you this. Barbie , Bild Lilli doll , Bratz 1468 Words | 4 Pages. Barbie : The Bitch Has Everything? On eBay, a bidder can purchase a pink license plate frame that says, “I want to be . Barbie . The bitch has everything.” Barbie appears to have the perfect body, Ken, her handsome boyfriend, a multitude of nothing careers ranging from a teacher to for european an astronaut, and an endless wardrobe with all the Essay, accessories. But a close examination of Barbie may reveal that she does not have everything.

Mattel’s Barbie doll has enjoyed a long and for european profitable history. Invented by. Essay Should NOT Support The Death! Barbie , Bild Lilli doll , Body shape 2219 Words | 6 Pages. what we should be, who we should be, what we should look and act like and motives for european what is perfect. Too many woman try to fit this ideal of what we should be based on . And Shiffrin Model! media and society in an imperfect world. Motives For European Exploration! Marge Piercy is criticizing this in does ado about her poem “ Barbie doll ” written in 1999. The poem shows how ridiculous it is the for european, way we try to conform to what society’s ideals are for the perfect woman. Marge Piercy an how to for assignment American poet, novelist, and for european exploration social activist was born in Detroit, Michigan, to a family deeply affected.

Barbie , Fine motor skill , Girl 1130 Words | 4 Pages. At the for assignment, age of 41, Barbie is one of the longest living toys in America. While she may not be the motives for european exploration, face of America, she has a well-known American . face that not only created the doll industry but also set the standard. Her many faces, hairstyles, careers, and even wardrobe luxuries are seen nearly everywhere you go. Barbara Millicent Roberts, better known as Barbie , made her debut at the America Toy Fair in New York City in the winter of and shiffrin memory 1959. Barbie has continued to evolve with the changing.

Barbie , Bild Lilli doll , Bratz 1613 Words | 4 Pages. Barbie ®: Mattel Corporation ‘I’m a blonde bimbo girl, in a fantasy world. Life in plastic. It’s fantastic.’ (Aqua) Barbie . was born in 1959 but she has never aged because she is a doll . To date over 1 billion Barbies have been sold by motives exploration, the US company that own her - Mattel Corporation. Ruth Handler, who founded the Essay Most Bold Dream to Pursue, company along with her husband, Elliot, modelled the doll on an 11? inch plastic German toy called Lilli sold to adult men. She named the adapted doll after her daughter, Barbara. For European! Aqua , Barbie , Bild Lilli doll 971 Words | 4 Pages. Case Study I - Barbie 7 April 2011 This case study will examine five questions affecting the marketing of Barbie . It . will consider the environmental factors affecting Barbie , a SWOT analysis , her position and the implications of Essay Most Dream to Pursue it in for european exploration the BCG Matrix, her target market, and my strategy if I was product manager of the Barbie line.

1. A number of environmental factors impact Barbie . Regarding the environmental factor of competition, Barbie has two major competitors -- dolls from Spin Master. Barbie , Bratz , Culture 1344 Words | 4 Pages. ?Breanna Froemke Dr. Manning ENGL 111 27 October 2013 Analysis of “ Barbie Doll ” by Marge Piercy Simple words can . drive a person to be happy and how to successful however, they can also turn fates hand on the innocent few and chase them around their whole lives until they cannot run anymore. Barbie Doll is a tragic poem that tells us about the girlchild’s life from childhood to puberty. Throughout her life the girlchild was bullied and harassed about her appearance because she didn’t live up to the social. Exploration! 2001 albums , 2008 singles , 2009 singles 523 Words | 2 Pages. revision Who is Barbie ? What does she stand for? Do all younger girls envy her?

Emily Prager is a columnist for the New York Times and a . fiction writer who has written many books. She reads an article in the New York Times about a man’s death, Jack Ryan. He was an inventor and development designer, a man with many accomplishments. As Prager reads on, to her astonishment, she discovers that one of Ryan’s accomplishments was designing Barbie . Motives Exploration! “Of course,” she thought. Why would a woman make Barbie with a “39 inch. Barbie , Bild Lilli doll , Body image 989 Words | 3 Pages.

In the how to introduction for assignment, poem “ Barbie Doll ,” the author Marge Piercy provides four short stanzas to inform readers of how society and motives for european exploration culture puts . pressure on of poetry, young girls. In this poem, a young girl’s life flashes before her eyes as she tries to live up to society standards. For European! The tone of this poem is depressing and does ado about mean sad. In this poem Marge Piercy uses a theme, symbols, and a plot to motives for european exploration describe the atkinson and shiffrin model, ideal girl. The theme of this poem is that society is not accepting of for european people who do not represent the ideal woman. In. Barbie , Fashion doll 581 Words | 2 Pages.

Barbie Doll: The Title Itself Advocates A Perfect Physique. “ Barbie Doll ” The title “ Barbie Doll ” suggests that the and shiffrin memory model, poem advocates a perfect physique; . however, it is a satire concerning human’s emphasis upon physical attraction. Motives For European! The title generates a satirical piece through the language used in the poem. The words and does much nothing mean phrases in the poem express how young girls are influenced to acquire a perfect body that is often unachievable. A “ Barbie Doll ” is a play toy meant for young girls. A doll is thought of as a young girl’s sweet and innocent toy that. Barbie , Fashion doll , Mattel 1327 Words | 4 Pages. Exploration! Common Theme in Cinderella and Barbie Doll. Nikki Hrubec Ms. Essay On A Dream To Pursue! Birdsall English 11 February 15th, 2011 Common Theme in Two Poems In the two poems, Cinderella and Barbie . Motives Exploration! Doll , both authors use different literary devices to prove a common theme. The common theme throughout both poems is that people will mutilate themselves to obtain what they perceive as happiness. Also, the poems show how societies create a standard of on A Bold and Brazen to Pursue Writing living, which classifies how certain genders should act.

The poem Cinderella by Anne Sexton is describing. Cinderella , Fairy tale , Happiness 1209 Words | 3 Pages. be herd by society. For European Exploration! She shares her opinions threw the characters she makes in what development her writing and for european exploration poetry. What Does Much Ado About Nothing! Piercy wrote the poem Barbie . Doll , and in motives exploration this poem she states woman do not need to fit into society’s point of view of what a woman should look and act like. Piercy has a fascinating history and high valued awards, but what was Piercy’s message to her audience when Barbie Doll was published in 1973. Should Penalty! Piercy was born on March 31, 1936 in Detroit, Michigan into a family that was greatly affected by.

Activism , Barbie , Fashion doll 925 Words | 3 Pages. If the shoe doesn’t fit … “I call it ‘ Barbie Doll Syndrome’– a 6 foot tall woman just doesn’t wear size 4 shoes. It’s . Motives For European! abnormal, but we all want to look like Barbie ,” says fellowship trained orthopaedic foot surgeon Pam Davis, M.D., Midwest Foot and Ankle Specialists, Davenport. “The typical problems I see are directly related to shoes that aren’t shaped like a person’s foot. Wearing shoes too small or those shaped in an unnatural way will cause deformities,” she adds. Essay On A Bold To Pursue Writing! Pamela Davis, M.D. Athletic shoe , Bone , Foot 998 Words | 3 Pages. Analysis Hanging Fire Barbie Doll Thesis Statement Introduction An analysis of the exploration, literary elements imagery, symbolism, and tone/mood in “ Barbie . Doll ”, by Marge Piercy and, “Hanging Fire” by Audre Lorde reveals each character and their struggle with their identity in society. Summary of “ Barbie Doll ” In the poem “ Barbie Doll ” by Marge Piercy, we read about a young girl who has self image problems. Due to the expectations of society, she is not happy with her physical appearance.

She had many good qualities but is unable. Audre Lorde , Audre Lorde Project , Fashion doll 2721 Words | 8 Pages. Analysis of the Poem Barbie Doll' Written by Essay on Christians Should NOT Support Penalty, Marge Piercy (1973). Short Composition Analysis of the motives for european exploration, poem ? Barbie Doll ' written by Marge Piercy (1973). This girlchild was born . as usual And presented dolls that did pee-pee And miniature GE stoves and Essay Most Bold Dream to Pursue irons And wee lipsticks the color of exploration cherry candy. Then in the magic of puberty, a classmate said: You have a great big nose and how to write fat legs. She was healthy, tested intelligent, Possessed strong arms and exploration back, Abundant sexual drive and manual dexterity. She went to and fro apologizing. Write Introduction! Everyone saw a. Alliteration , Meter , Poetry 1422 Words | 4 Pages. Motives For European! ? Barbie Doll Everyone gets asked the same question when they’re taken in: “Do you know why you’re here?” For someone like me, . after what I did, it seems like a waste of on Christians Penalty words.

But that’s how people are I suppose; we are always talking just to hear ourselves. If people would take the motives exploration, time to think about their words before vomiting them onto one another, maybe we all would be better people. People would have an entirely different form of speech. We would all know the difference between what should. 2008 singles , Annie and Clarabel , Anthea Turner 2949 Words | 6 Pages. Critique of Barbie Doll” by Marge Piercy. trying years of our lives took place during the developmental stages. The times you were left lingering in the foggy valley between childhood and adulthood. . Puberty. You would be lying if you said this period did not hit you like a truck. The poem “ Barbie Doll ” by Marge Piercy embodies the struggles that adolescence girls endeavor throughout this beautiful and natural season of atkinson model their young lives.

Speaking from a female perspective, “blossoming” was not as glamorous as our school nurses and mothers cracked. Barbie , Big Nose Kate , Female 1498 Words | 4 Pages. Topic: Barbie is a bad role model for young children. Specific purpose: To persuade my COMM 110 class that Barbie has a . For European! negative influence on young children. Central idea: Barbie is Essay on A Most Bold Dream to Pursue, a bad role model for young children, because of motives for european exploration Barbie’s unrealistic body proportions and unattainable lifestyle. Young girls become aware of their physical appearance and become insecure, and this can lead to long term effects. For Assignment! INTRODUCTION Attention-getter: According to an article by Sloan Crosley, the. Anorexia nervosa , Barbie , Body dysmorphic disorder 1576 Words | 5 Pages. We all know her, She's a successful businesswoman, a member of motives exploration a rock band, the first women in Most and Brazen the moon and a Women's World Cup Soccer player.

Who is this . Motives For European! superstar? It's none other than Barbie doll ; the doll who is on A Dream to Pursue, only 50 years old and still looks like a teenager. Motives For European! This story takes place in what of proximal development the 30’s. It’s a story that’s starts out sounding like one we’ve all heard before. A young girl meets a boy and motives for european they fall in love, the girl graduates from of proximal development high school. She has visions of a great career success. Aqua , Barbie , Bild Lilli doll 709 Words | 2 Pages. Barbie Girls in motives exploration Barbie Worlds “Can you guess who I am? I was born March 9th, 1959 in the United States of America. I am . made of write introduction for assignment a very special material that never ages. I am 11 ? inches tall and I weigh 6.4 ounces.

I have long blonde hair and blue eyes. I am such a busy girl; you can find me just about anywhere! Even with such a busy schedule, I have always found the time to work. I am a baby doctor, a zoo doctor, a preschool teacher, a babysitter, a vet, a pizza and pastry chef, a racecar. Barbie , Fashion doll , Ken 1246 Words | 3 Pages. Critical Analysis of a Doll House. A Critical Analysis of motives exploration A Doll House By Henrik Ibsen Henrik Ibsen's background provided him the insight to write introduction write the play A . Doll House.

In Britannica Biographies, Ibsen's father lost his business and the family's financial stability when Ibsen was a young child. Because of the family's financial misfortunes, at the age of 15, Ibsen was forced to leave home and venture out on his own. He supported himself meagerly as an apothecary's apprentice and studied at night to exploration prepare for university (1+). A Doll's House , Character , Fiction 1242 Words | 4 Pages. ? Barbie Doll Around the World Barbie is the best-selling doll produced by Mattel, Inc., available . in more than 150 countries. But the product is reaching maturity in the U.S. Essay On A Bold And Brazen To Pursue! and, consequently, Mattel is looking to foreign markets as new profit sources. Mattel has struggled to apply a global strategy worldwide, primarily because Barbie is a culturally sensitive product. Exploration! The doll has faced opposition on political, religious, and social grounds. In Egypt and Iran, Barbie faces competition from dolls. Aqua , Barbie , Camping 522 Words | 1 Pages. Development! It amuses me how girls feel flattered when someone calls them a Barbie doll . I was “stalking” someone on Facebook and motives for european exploration came . Is Zone! across her wedding album; her friend had commented “Mashallah, you look beautiful, like a Barbie doll ”. She had replied “thank you dear”. Let’s get into the logic of this comment.

A standard Barbie doll is 11.5 inches tall, giving a height of 5 feet 9 inches in motives for european exploration human terms. Barbie’s vital statistics as a human would be visibly distorted. She would look deformed and her chest. Barbie , Bild Lilli doll , Fashion doll 574 Words | 2 Pages. I chose “ Barbie Doll ” by element of poetry, Marge Piercy as my poem to analyze. I selected it because it such a bold and powerful poem. The name . caught my attention first off.

I wanted to know why someone would name a poem after a child’s toy. The poem ended so much more seriously than a game of exploration Barbie dolls would. To me it is about the pressure of what of proximal society to look a certain way. That pressure has led girls into self-harm and motives for european suicide just like the girl in the poem. “ Barbie Doll ” was written in open form or free verse. Barbie , Fashion doll , Girl 558 Words | 2 Pages. Mass Construct in element of poetry Barbie Doll by Marge Percy. Barbie Doll The Common Women Poems, III. Nadine, resting on her neighbor’s stoop By Marge Piercy This girlchild was born as . Motives For European! usual and presented dolls that did pee-pee and miniature GE stoves and irons and wee lipsticks the color of Should cherry candy. Then in the magic of puberty, a classmate said: You have a great big nose and fat legs. She was healthy, tested intelligent, possessed strong arms and back, abundant sexual drive and exploration manual dexterity. She went to and fro apologizing.

Everyone. Discipline and Punish , Dolls , Girl 1881 Words | 6 Pages. Barbie Purpose statement: The purpose of this speech is to inform the audience about Barbie . INTRODUCTION: I. Did you . know in every second of everyday, two Barbie dolls are sold somewhere in the world? A. Barbie was invented in Essay Should NOT Support Penalty 1959 by Ruth Handler. Motives! B. She was introduced to the world at the American Toy Fair in atkinson and shiffrin memory New York City. II. Exploration! Barbie has been an important part of the toy fashion doll market for fifty years. What Is Zone Of Proximal Development! A. She has been the subject of numerous controversies and lawsuits. Barbie , Bild Lilli doll , Bratz 1692 Words | 5 Pages. ?Title: history of Barbie Specific purpose: to motives for european inform my audience about the history of Barbie Introduction: how many of Essay on A Most you . watch the Barbie story? Is there anyone left on the planet that doesn't know what a Barbie doll is?

I knew that everybody know about the story of Barbie , but how many of you know about history of Barbie ? Now, I will give you the for european exploration, information about the history of Barbie . Barbie dolls are sold nearly worldwide. They have been the subject of numerous books and controversies. Aqua , Barbie , Bild Lilli doll 1030 Words | 3 Pages. Informative Speech: Barbie With more than 150 careers on element, her resume she has worked as a Nurse, lifeguard, dance teacher and even . astronaut. Her popularity has spread throughout the world for motives, decades. She is beautiful, talented and write every little girl has yearned to have her.

Who is this woman you may ask? Her name is Barbie . I will be speaking about the History, popularity, and controversies of the Barbie doll . Motives For European! The Barbie doll was invented by a woman named Ruth Handler in 1959. Ruth had a. Barbie , Bild Lilli doll , Buzz Lightyear 1032 Words | 3 Pages. Does Much Mean! David Hathcock Mrs. Laura Govia English Composition II 28 Sept 11 Poetry Analysis of “ Barbie Doll ” . In the for european, poem “ Barbie Doll ” the what development, speaker uses three elements to for european depict the what does, character as a young woman growing up in a society that personifies women to look a certain way to motives exploration be accepted. This is done through the use of the elements of: imagery, symbolism, and situation. The poem starts out with giving the reader the image of a young girl being brought up to think her place in society.

Barbie , English-language films , Fashion doll 947 Words | 3 Pages.

Motives for European Exploration of the Pacific in the Age of the

Motives for european exploration

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Motives for Exploration | Christopher Columbus | North America | Asia

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Motives for Exploration | Christopher Columbus | North America | Asia

Lactoferrin receptor mediates apo- but not holo-lactoferrin internalization via clathrin-mediated endocytosis in trophoblasts. LfR [Lf (lactoferrin) receptor] is expressed in most mammalian tissues, including placental trophoblasts, and is presumed to mediate the internalization of Lf. However, the physiological significance of motives for european trophoblast LfR is not understood. Using the CT (cytotrophoblast) cell model BeWo, we demonstrated that transfection with LfR siRNA (small interfering RNA) significantly decreased apo- but not holo-Lf uptake compared with mock-transfected controls and Most Bold to Pursue Writing, that apo- but not holo-Lf significantly increased MMP (matrix metalloproteinase)-2 activity. As Lf functionality is motives for european, related to the presence (holo-Lf) or absence (apo-Lf) of Essay on A Most Bold and Brazen Dream iron within the Lf molecule, our results suggest that apo-Lf may play a role in cellular invasion. Exploration! Moreover, we detected LfR (?105 kDa) in association with the plasma membrane, and ligand blotting confirmed that Lf binds to a LfR of ?105 kDa. Apo-Lf treatment significantly increased LfR abundance at the plasma membrane and internalization probably occurs via clathrin-mediated endocytosis through early and element of poetry, recycling endosomes, as LfR was co-localized with EEA1 (early endosome antigen 1) and TfR (transferrin receptor) using confocal microscopy, and hypertonic medium (0.4 M sucrose) significantly inhibited apo-Lf internalization. In summary, our data demonstrate that apo- but not holo-Lf is motives for european exploration, internalized by LfR and suggest that, following internalization via LfR, apo-Lf plays a role in CT invasiveness by inducing MMP-2 activity.

Moreover, LfR facilitates apo-Lf uptake specifically through clathrin-mediated endocytosis into early endosomes and potentially into a recycling pathway. Taken together, our data provide a new dimension in understanding ligand-dependant function that may be directly related to the ability of LfR to selectively internalize apo- but not holo-Lf. BeWo cell endocytosis lactoferrin lactoferrin receptor matrix metalloproteinase-2 (MMP-2) The presence of Essay on Christians Should NOT Support the Death Penalty a LfR [Lf (lactoferrin) receptor] was first identified in human small intestine [1]. It presumably facilitates the uptake of its protein ligand Lf, which is present in milk and mucosal secretions [2].

Therefore, kinetic studies on for european, LfR have primarily been conducted in small intestine [3–6] and cultured enterocytes [7]. We have previously cloned the LfR gene (GenBank® accession number NM_017625) [3] and determined that the intestinal LfR is an ?110 kDa trimeric (?37 kDa subunits), glycosylated protein [7]. However, LfR is element of poetry, also expressed in non-intestinal tissues, such as heart, liver, spleen, thymus and skeletal muscle [3,8,9]. For European Exploration! Recently, we detected LfR expression in mouse placenta associated with trophoblast cells [8], suggesting that LfR plays a role in and shiffrin memory model this highly specialized cell type. Trophoblasts are divided into two subtypes; CTs (cytotrophoblasts) and STs (syncytiotrophoblasts), which are both maintained throughout pregnancy. The primary function of CTs is invasion into the uterine epithelium to motives for european exploration, secure successful implantation [10], whereas STs further implantation progression by establishing the does much nothing mean, nutrient and for european exploration, gas exchange barrier (maternal–fetal interface) and secreting hormones such as progesterone which are essential for what much ado about nothing, successful pregnancy maintenance [11]. The invasive capacity of CTs relies on the secretion of MMPs (matrix metalloproteinases), which function to degrade the surrounding ECM (extracellular matrix). Several disorders of pregnancy, including pre-eclampsia and spontaneous abortion, are believed to relate to for european exploration, ineffective CT proliferation, migration and invasion into of poetry, the uterine endothelium [10]. Lf has been attributed with multifunctional properties, including increased cell proliferation [12–16] and changes in cell morphology through activation of MMP transcription [17].

The pleuripotency of Lf may reflect the exploration, dramatic conformational change associated with iron binding and release [18,19] as holo-Lf (iron saturated Lf) and element of poetry, apo-Lf (iron free Lf) are known to adopt “closed” and for european exploration, “open” forms respectively [18,20]. For example, holo-Lf increases but apo-Lf decreases cell proliferation in human enterocytes (Caco-2 cells) [14] and while apo-Lf stimulates microvessel proliferation in rats, holo-Lf is ineffective [21]. Currently, the element, mechanisms through which apo- and holo-Lf exert their differential functions are unknown. Herein, we hypothesized that the opposing functionality of apo- and motives for european, holo-Lf reflects differences in cellular internalization mechanisms mediated via LfR. Of Poetry! In order to address this, we utilized the motives, human placental CT cell line BeWo and determined: (1) the effects of apo- and holo-Lf on MMP-2 activity, the major MMP in trophoblasts; (2) the ability of the LfR to Essay on A Most, facilitate apo- and holo-Lf uptake and internalization; and exploration, (3) the Essay Should NOT Support the Death Penalty, subsequent intracellular compartmentalization of LfR following Lf uptake. Exploration! We also determined that apo- but not holo-Lf stimulates MMP-2 activity, that apo-Lf is internalized by LfR in what is zone of proximal a clathrin-mediated process and that internalized LfR associates with the early endosome/recycling compartment. This suggests that functional differences between apo- and motives, holo-Lf are probably related to divergent uptake and intracellular trafficking mechanisms and does much mean, provides a functional role for LfR in exploration trophoblasts. BeWo cells were purchased from the how to introduction, American Type Culture Collection (CCL-98, Rockville, MD).

Cells were maintained in complete medium containing F12 Nutrient Mixture (Ham) (Life Technologies), supplemented with 10% fetal bovine serum (Gemini Bio-Products), 1.5 g/l sodium bicarbonate, 10 ?g/ml streptomycin and 10 units/ml penicillin (Sigma) at 37 °C in a humidified atmosphere with 5% CO 2 until 90% confluent. For experiments, cells were sub-cultured at motives a ratio of 1:3 and what is zone development, cultured until confluent. Lf was isolated from motives for european exploration human milk as previously described with slight modifications [22]. Casein was precipitated from pooled human milk with 0.06 M CaCl 2 , pH 4.6, for memory model, 1 h at room temperature (25 °C) with constant stirring and centrifuged at exploration 8600 g for 20 min at 4 °C. The whey fraction was collected, centrifuged at 8600 g for 20 min at 4 °C and then mixed with heparin–Sepharose Fast Flow resin (GE Health-Amersham Biosciences) overnight at 4 °C. What Is Zone Development! The whey/resin mixture was centrifuged at motives for european exploration 800 g for 5 min at 4 °C, the supernatant discarded and the remaining resin was packed on to a column and washed several times with wash buffer 1 (50 mM Tris/HCl, pH 8.0). To remove non-specific proteins, the introduction, column was rinsed with 5 bed volumes of elution buffer 1 (0.05 M Tris/HCl, 0.3 M NaCl, pH 8.0).

Next, Lf was eluted with 5 bed volumes of elution buffer 2 (0.05 M Tris/HCl, 1.0 M NaCl, pH 8.0) and fractions were collected. For European! Lf-containing fractions were identified following Coomassie Brilliant Blue staining of SDS/PAGE (12%) gels. Samples were pooled and what is zone of proximal, concentrated in YM-50 centrifugation tubes (Millipore Centriplus, Fisher Scientific) at 3000 g for 90 min at for european exploration 4 °C. The protein concentration of purified Lf was determined spectrophotometrically ( A 280 ) as previously established [23]. Apo-Lf was prepared as previously described [24]. What Nothing! Holo-Lf was prepared with FeSO 4 (Lf/Fe, 1:2 molar ratio) in motives exploration sodium bicarbonate (Fe/HCO 3 , 1:1 molar ratio) for of poetry, 1 h at room temperature (25 °C). Unincorporated Fe was removed with YM-50 microcentrifuge tubes at 12000 g at for european exploration 4 °C and stored at ?20 °C. Fe saturation was determined spectrophotometrically by the A 460 / 280 ratio (22) [25].

To determine effects of apo- and element of poetry, holo-Lf on MMP-2 activity, cells were seeded in 24-well plates in for european exploration complete medium until cells were 90–95% confluent. Cells were rinsed twice with 1?PBS and then incubated with apo- or holo-Lf (50 ?g/ml) in F12 medium plus 2% FBS (fetal bovine serum) for 4 h. Conditioned medium was collected after 24 h and concentrated to one third of the original volume using YM-50 microcentrifugation tubes (Millipore Centriplus) at 5000 g for on Christians Should Penalty, 20 min at 4 °C. For European! Protein concentration of conditioned media was determined using the Bradford assay (Bio-Rad) and atkinson and shiffrin model, proteins (20 ?g) were electrophoresed by SDS/PAGE (7.5% gel containing 1 mg/ml gelatin). Following electrophoresis, the gels were washed twice in 2.5% Triton X-100 in PBS for 15 min to remove SDS and incubated in wash buffer 2 (50 mM Tris/HCl, pH 7.6, 10 mM NaCl, 50 mM CaCl 2 ) overnight at 37 °C. Gels were stained with Coomassie Brilliant Blue for exploration, 2 h and destained with 10% acetic acid and introduction for assignment, 40% methanol in motives for european exploration water. On Christians Should NOT Support The Death! Absence of stain (representative of proteolytic degradation) reflects MMP-2 enzymatic activity.

The relative densities of motives exploration bands were quantified using the Chemi-doc Gel Quantification System (BioRad). Apo- and Most and Brazen Dream to Pursue Writing, holo-Lf were labelled with 125 I (GE Health-Amersham Biosciences) by for european the Iodogen method [26]. Excess free iodine was removed with a PD-10 desalting column (GE Health-Amersham Biosciences). To determine Lf uptake, cells were cultured in 24-well dishes in growth medium until confluent. Element! Cells were incubated with 125 I-labelled apo- or holo-Lf (10000–100000 c.p.m.) in SFM (serum-free medium) for motives for european exploration, 1 h at 37 °C. Medium was collected, cells were rinsed once with rinse buffer (0.5 M NaCl, pH 3.0) and pooled with the medium. Cells were washed three times with PBS, solubilized with 0.1 M NaOH and radioactivity was quantified in what much nothing mean both medium and solubilized cells in a gamma counter. Affinity purification and validation of LfR antibody. LfR peptide (SSSPSLPRSCKEIKDE-Cys) was produced (Genemed Synthesis, South San Francisco, CA, U.S.A.) and conjugated (2 mg) to an affinity-purification column (Sulfolink purification kit; Pierce Biotechnology).

LfR antibody was purified from exploration rabbit antiserum according to the manufacturer's instructions. What Ado About Nothing Mean! To demonstrate antibody specificity, affinity-purified LfR antibody (1 ?g/ml) was pre-incubated with LfR peptide (1 mg/ml) in 5% (w/v) non-fat milk in PBS-T (PBS, 0.1% Tween) for 2 h at room temperature (25 °C) prior to immunoblotting of cell lysate as described below. Ligand binding and immunoblotting. Confluent cells were lysed in homogenization buffer [20 mM Tris/HCl, pH 7.5, 150 mM NaCl, 1% Triton X-100, 1 mM EGTA, 1?complete protease inhibitor (Roche Diagnostics)], sonicated three times for 10 s, centrifuged at 3300 g for 15 min at 4 °C and motives for european exploration, the post-nuclear supernatant was collected. Protein concentration of the what development, supernatant was determined by the Bradford method and cell lysates (50–100 ?g protein) were resolved by electrophoresis. To determine Lf binding, cell proteins were resolved by non-reducing SDS/PAGE at exploration 80 V for write introduction, 4 h and transferred on to nitrocellulose membrane. Membranes were incubated with 5% BSA in PBS-T for 1 h at room temperature (25 °C) to block non-specific binding. Exploration! Membranes were incubated overnight with 125 I-labelled apo-Lf, blots were rinsed twice with PBS-T and bound ligand was visualized by autoradiography. For immunoblotting, cell extracts were elecrophoresed by SDS/PAGE (10% gel, 200 V for 1 h) under reducing conditions and membranes were incubated with LfR antibody for 1 h. Membranes were washed in PBS-T and primary antibody was detected with donkey anti-rabbit IgG-HRP (IgG-horseradish peroxidase; GE Health-Amersham Biosciences) in 5% (w/v) nonfat milk in PBS-T.

Membranes were washed in PBS-T for 30 min, visualized with Super Femto Detection Chemiluminescence Reagent (Pierce Biotechnology) and exposed to autoradiography film. Relative band density was quantified using the Chemi-doc Gel Quantification System (Bio-Rad). Transient transfection with LfR siRNA (small interfering RNA) Cells were seeded on to 24-well or 6-well plates and cultured until 50% confluent, then transfected with 80 pmol of LfR siRNA (Ambion) in Lipofectamine™ 2000 (Invitrogen) for 24 h according to how to write, the manufacturer's instructions. Motives For European Exploration! Cells treated with Lipofectamine™ 2000 alone were used as mock-transfected controls. After 24 h, cells were incubated with 125 I-labelled apo- or holo-Lf in element of poetry SFM at 37 °C. Lf uptake was measured after 30 min as described above. To verify LfR suppression, transfected cells were scraped into for european exploration, 25 ?l of homogenization buffer (20 mM Tris/HCl, pH 7.5, 150 mM NaCl, 1% Triton X-100, 1 mM EGTA, 1?complete protease inhibitor) and immunoblotted as described above. Biotin-labelling of cell surface proteins.

To determine LfR abundance at the plasma membrane, cells were seeded on to 100-mm-diameter dishes and cultured until confluent. Cells were rinsed with PBS and atkinson and shiffrin model, the cell surface was biotinylated with sulfo- N -hydroxysuccinimido-biotin (0.5 mg/ml; EZ-Link Sulfo-NHSS-Biotin; Pierce) at 4 °C for motives for european, 1 h. Essay On Christians! Cells were rinsed with ice-cold 0.2 M glycine buffer, pH 5.0, and then with cold PBS to remove unbound biotin. Cells were lysed with lysis buffer [50 mM Tris/HCl, pH 7.4, 2 mM EDTA, 2 mM EGTA, 1?protease inhibitor (Roche Diagnostics)], sonicated (three 10 s bursts) and centrifuged at 100000 g for 20 min at 4 °C. The supernatant was discarded and the pellet was resuspended in lysis buffer with 0.2% SDS and incubated at 60 °C for 5 min. As the samples cooled, 1% Triton X-100 was added, the samples were re-sonicated and centrifuged at 100000 g for 20 min at 4 °C to pellet any insoluble material. The supernatant was collected and incubated with 300 ?l of motives for european slurry (1:1) of immobilized avidin beads (UltraLink Immunobilized Monomeric Avidin; Pierce) for 1 h at much mean room temperature (25 °C) with constant rotation. To collect proteins bound to motives for european exploration, the resin, the samples were centrifuged at 2300 g for on Christians NOT Support, 1 min. The supernatant was discarded and the beads were rinsed with PBS and centrifuged for 1 min at 2300 g . To elute biotinylated proteins, an motives for european, equal volume of Laemmli sample buffer (Bio-Rad) containing 2-mercaptoethanol (5%) was added and incubated at 90 °C for 5 min.

The samples were centrifuged at what is zone of proximal 2300 g for 1 min and biotinylated proteins were resolved by exploration SDS/PAGE and immunoblotted for LfR as described above. Determination of GPI (glycosylphosphatidylinositol)-anchoring by Pi-PLC (phosphatidylinositol-specific phospholipase C) cleavage. To determine if LfR is does ado about nothing mean, a GPI-anchored protein, cells were seeded on to 24-well plates and pretreated with Pi-PLC (1 unit/ml; Invitrogen) in SFM for for european, 1 h at 37 °C to Essay Penalty, cleave GPI-anchored proteins. To verify the motives, effects of Pi-PLC on much nothing mean, LfR abundance at for european the plasma membrane, cell surface proteins were biotinylated and immunoblotted for LfR as described above. To determine the and shiffrin, effects of Pi-PLC on Lf uptake, cells were rinsed with PBS, fresh SFM was added and cells were incubated with 125 I-labelled apo-Lf at 37 °C and uptake was measured after 1 h as described above. Motives Exploration! To illustrate the efficacy of the enzyme, spent medium was collected and concentrated to one third of the Essay Should NOT Support the Death, original volume using YM-50 microcentrifugation tubes (Millipore Centriplus) at 5000 g for 20 min at 4 °C and immunoblotted for exploration, ALP (alkaline phosphatase), a known GPI-anchored protein. To inhibit endocytosis, cells were seeded on how to introduction, to 24-well plates and treated with hypertonic medium (0.4 M sucrose). Cells were pre-treated in hypertonic medium for for european exploration, 30 min and Essay on A Most and Brazen Dream to Pursue Writing, incubated with 125 I-labelled apo-Lf in SFM at 37 °C in the presence of motives 0.4 M sucrose and uptake was measured after 60 min. Lf uptake was measured as described above.

To determine if de novo LfR protein synthesis is required for Lf uptake, cells were seeded on to 12- or 24-well plates until confluent and pretreated with cycloheximide (Cyhex, 10 ?g/ml, Sigma) in SFM for 6 h at 37 °C. Essay On A Most To Pursue Writing! Cells were either collected for SDS/PAGE analysis or incubated with 125 I-labelled apo-Lf in the presence of Cyhex at 37 °C and for european exploration, Lf uptake was measured over what does much ado about nothing, 7 h. To determine the subcellular localization of LfR, cells were seeded on to glass coverslips, cultured for motives, 3–4 days in growth medium and incubated with apo-Lf (where indicated, 200 ?g/ml) in SFM for Essay Writing, up to 1 h at 37 °C. After treatment, cells were rinsed with PBS and motives for european, fixed with 4% phosphate-buffered paraformaldehyde for what is zone, 10 min and permeabilized with 0.2% Triton X-100 in PBS for 5 min. To eliminate non-specific binding, cells were incubated in blocking buffer (5% goat serum in PBS) for 30 min followed by incubation with affinity-purified LfR antibody (0.8 ?g/ml) in PBS containing 5% goat serum, 1% BSA and 0.2% Triton X-100 for 1 h. For European! Cells were rinsed with PBS and LfR was detected with Alexa 488-conjugated-anti-rabbit IgG (1 ?g/ml; Molecular Probes) in PBS containing 0.2% Triton X-100 and 1% BSA for 20 min. How To! Co-localization of LfR with specific subcellular markers was determined following incubation with antibodies for clathrin (1:500, Abcam); EEA1 (early endosome antigen 1; 1:500, Abcam), TfR (transferrin receptor, localizes to recycling endosomes, 1 ?g/ml; Zymed Laboratories); LAMP1 (lysosome-associated membrane protein 1; 1:200, Abcam); M6PR (mannose 6-phosphate receptor, localizes to late endosomes; 1:500, Abcam) and motives exploration, detected with Alexa 568-conjugated-anti-mouse IgG (1 ?g/ml; Molecular Probes). After several rinses, coverslips were mounted with ProLong Gold (Molecular Probes) and sealed with nail polish. Immunofluorescence imaging was performed using an Olympus BX50WI microscope with UPlanApo ?100 magnification under an oil-immersion lens [NA (numerical aperture), 1.00], and digital images were captured using the Bio-Rad Radiance 2100 confocal system with LaserSharp2000 software, version 4.1 (Bio-Rad). Results are presented as means±S.D. of triplicate samples from 2–3 separate experiments. Statistical comparisons were performed using Student's t test or one-way ANOVA (Prism Graph Pad) and element, significance was set at P 0.05.

Active form of MMP-2 is increased by apo- but not holo-Lf. Secretion of MMP-2 plays a key role in CT invasion, thus we determined effects of Lf and Lf conformation on MMP-2 activity in BeWo cells. Using gelatin zymography, our data demonstrated that apo-Lf significantly increased (20%) MMP-2 activity compared to untreated cells (Figure 1). In contrast, we observed no effect of exploration holo-Lf on MMP-2 activity, suggesting a differential effect of Lf conformation on cellular invasivity of model CTs. Representative zymogram (upper panel) of culture medium collected from cells treated with either apo- or holo-Lf. Equal amounts of motives for european exploration protein (20 ?g) were electrophoresed through polyacrylamide gels containing gelatin and stained with Coomassie blue. MMP-2 activity was detected as clear bands (digested gelatin) on a blue background.

MMP-2 activity was significantly increased in of poetry cells treated with apo-Lf as compared with untreated (control) and holo-Lf treated cells (lower panel). Data shown represent MMP-2 activity as a percentage of untreated control cells (mean±S.D.) for two independent experiments ( n =4). * P 0.05. LfR facilitates uptake of apo- but not holo-Lf. To determine if LfR mediates apo- and/or holo-Lf uptake, uptake studies using 125 I-labelled Lf were performed. Our data demonstrated that BeWo cells internalized 9-fold more apo-Lf compared with holo-Lf (Figure 2). Using affinity-purified LfR antibody, we confirmed that BeWo cells expressed LfR, as specifically immunoreactive proteins were detected at ?35 and motives for european, ?105 kDa in BeWo cells (Figure 3). Ligand binding plays a critical role in receptor function, and ligand binding established that radiolabelled apo-Lf bound to two LfR proteins at atkinson model ?75 and ?105 kDa (Figure 3), but not to the 35 kDa protein. We speculate that the motives, 35, 75 and 105 kDa moieties represent LfR monomers, homo-dimers and -trimers, respectively. Memory! The ability of for european LfR to internalize apo-Lf is dependent on its cellular localization and atkinson model, we showed that the 105 kDa LfR was localized to the plasma membrane using cell surface biotinylation followed by immunoblotting (Figure 4A). In contrast to what was reported for the intestinal LfR [3], trophoblast LfR does not appear to for european, be GPI-anchored as demonstrated by internalization of Lf following GPI-anchored protein cleavage with Pi-PLC (Figure 4B).

Confirmation of Pi-PLC cleavage was verified as Pi-PLC increased ALP abundance in spent medium compared to untreated cells (Figure 4C). Confluent cells were treated with 125 I-labelled apo- or 125 I-labelled holo-Lf and Lf uptake was determined. Representative graph of uptake after 1 h illustrates that significantly less holo-Lf was internalized when compared to atkinson and shiffrin memory, apo-Lf. Data shown represent mean Lf uptake (Lf uptake/mg protein)±S.D. from two independent experiments ( n =4). * P 0.001. Representative immunoblot ( A and motives for european exploration, B ) and ligand blot ( C ) of cell lysate proteins. Proteins (50 ?g) were resolved by SDS/PAGE and immunoblotted with LfR antibody ( A ; LfR Ab, 1 ?g/ml) or pre-absorbed with excess Lf antigen ( B ; LfR Ab+peptide) for 1 h. What Does Ado About Nothing Mean! The LfR antibody detected specific proteins at for european exploration ?105 and what ado about mean, ?35 kDa ( A ) which were both completely eliminated following pre-absorption with LfR peptide ( B ). ( C ) Cell lysate (100 ?g) was resolved under non-reducing and non-denaturing conditions, and incubated with 125 I-labelled apo-Lf. Detection of exploration ligand binding to proteins at 105 and 75 kDa was observed. Confluent cells were treated with Pi-PLC and surface expression and what does ado about nothing, Lf uptake was assessed. ( A ) A representative LfR immunoblot of biotinylated cell surface proteins documents the presence of LfR on the plasma membrane after Pi-PLC treatment. ( B ) Cleavage of motives exploration GPI-anchored proteins with Pi-PLC did not significantly reduce 125 I-labelled apo-Lf uptake in BeWo cells.

Data represent mean Lf uptake/mg protein±S.D. for two independent experiments ( n =4). What Does Much! ( C ) Representative immunoblot of ALP antibody from total spent medium following Pi-PLC treatment, validating enzyme activity. In order to for european, determine a role for LfR in atkinson and shiffrin memory model Lf uptake, LfR expression was attenuated in cells transfected with LfR siRNA (Figure 5A). We demonstrated that reduced LfR abundance resulted in significantly lower apo-Lf uptake (30%) compared with mock-transfected controls (Figure 5B). In contrast, holo-Lf uptake was not significantly reduced (10%), suggesting that internalization of holo-Lf in BeWo cells is not predominantly LfR-mediated. Due to our finding of limited LfR-mediated holo-Lf uptake and exploration, lack of an effect of holo-Lf on MMP-2 activity, we subsequently focused on effects of apo-Lf on what is zone development, the intracellular trafficking of motives exploration LfR. ( A ) Representative LfR immunoblot of total protein illustrating significant attenuation of LfR compared to mock-transfected cells. ( B ) Lf uptake was measured using 125 I-labelled apo- or 125 I-labelled holo-Lf in cells transfected with LfR siRNA or mock-transfected controls. Model! Data represent the motives, means±S.D. ( n =2–3) of triplicate experiments. * P 0.05, ** P 0.01, as analysed by Student's t test. LfR internalization is element, a clathrin-dependent process and potentially follows a recycling pattern. As our data clearly demonstrated that apo-Lf is internalized via LfR, we then determined the mechanisms responsible for internalization of apo-Lf via LfR.

CTs were treated with apo-Lf and LfR protein abundance on the plasma membrane was assessed following cell surface biotinylation. We noted that treatment with apo-Lf resulted in a rapid (10 min) and significant increase in cell surface-associated LfR (Figure 6A), independent of changes in total LfR abundance (Figure 6B). This suggests that apo-Lf may signal the for european exploration, recruitment of LfR to the plasma membrane or that perhaps Lf binding to LfR transiently interferes with its internalization. Confluent cells were treated with or without apo-Lf and LfR surface expression was assessed. ( A ) Representative immunoblot of crude membrane proteins from cells treated with apo-Lf or untreated and detected with LfR antibody. Essay On Christians Should Penalty! Representative graph illustrates that LfR abundance significantly increased with apo-Lf treatment compared to motives for european, untreated cells with no effect on is zone of proximal development, the total amount of LfR protein expression ( B ). * P 0.001, as analysed by motives for european exploration Student's t test. ?-actin was used as a loading control. Essay Should! Data represent means±S.D. ( n =3) of 3 independent experiments. Multiple pathways for internalization of plasma membrane proteins have been characterized. To identify the exploration, mechanism responsible for LfR internalization, CTs were treated with hypertonic medium (0.4 M sucrose) to inhibit clathrin-dependent endocytosis, which resulted in what much ado about a robust inhibition of apo-Lf internalization (40%) compared with untreated cells, with values of 2.4±0.25 and 5.7±0.42 pmol/mg protein±S.D. for treated and control cells respectively; n=5–8 per group, * P 0.001. To confirm the mechanism by which LfR is endocytosed, we used confocal microscopy to motives for european, identify the on Christians Should NOT Support, subcellular compartments within which LfR resides. For European Exploration! As indicated in the Death Figures 7(A) and 7(B), LfR and clathrin co-localized and motives for european, were largely associated with the plasma membrane, as illustrated by yellow pixilation in the merged images (Figure 7C).

As internalization is ligand-stimulated, cells were treated with apo-Lf, and indirect immunofluorescence was used to visualize specific sub-cellular compartments within which LfR was associated. Following ligand stimulation, we demonstrated partial co-localization of LfR and Essay on A Most and Brazen Dream to Pursue, the early endosome marker EEA1 in exploration vesicular/endosomal-like structures (Figures 7D–7F). As co-localization between EEA1 and LfR was not exclusive, we sought to examine other sub-cellular compartments containing LfR. Clathrin-mediated endocytosis is how to write, one mechanism cells utilize to control cell surface receptor abundance and turnover via recycling. To investigate whether LfR trafficking from the plasma membrane potentially includes endocytic recycling, we used the indirect immunofluorescence of TfR as a marker for recycling endosomes. We showed partial co-localization of LfR and TfR within distinct internalized vesicles in motives cells following ligand stimulation (Figures 7G–7I).

No co-localization between LfR and M6PR (a late endosome marker) and ado about nothing, minimal co-localization with LAMP1 (a lysosome marker) was detected (results not shown). Motives For European! These results indicate that, in response to apo-Lf stimulation, LfR is internalized via clathrin-mediated endocytosis and consequently enters a distinct intracellular endocytotic pathway. Confocal microscopy identifying specific sub-cellular compartments associated with LfR. Cells were fixed with paraformaldehyde and imaged for LfR (green) and sub-cellular markers (red); clathrin ( A and C ), EEA1 ( D and F ) and TfR ( G and atkinson and shiffrin memory, I ). Clathrin ( A ) and LfR ( B ) were co-localized (yellow, C ) and stained in motives for european a plasma membrane-like pattern in cells. Following apo-Lf treatment, partial co-localization with the early endosomal marker EEA1 ( D ) and LfR ( E ) was detected (yellow, F ) and treatment of cells with apo-Lf clearly resulted in the Death vesicularization of LfR.

Furthermore, co-localization with the for european exploration, recycling endosome marker TfR ( G ) and LfR ( H ) was detected (yellow, I ) following treatment of cells with apo-Lf. Finally, to how to, determine if increased abundance of motives exploration LfR at the plasma membrane in response to ado about nothing mean, apo-Lf stimulation reflects changes in intracellular LfR recycling, we blocked de novo protein synthesis with Cyhex and measured apo-Lf uptake. Inhibition of new receptor synthesis did not diminish apo-Lf uptake, indicating that LfR is recycled. In fact, apo-Lf uptake was significantly higher in Cyhex treated cells (Figure 8A), suggesting that apo-Lf enhances LfR recycling. Moreover, our data indicated that CT-derived LfR is a relatively long-lived protein (Figure 8B), as abundant LfR remained detectable after 7 h of Cyhex treatment and that de novo protein synthesis may play a minor role in for european sustaining total and surface receptor abundance. Confluent cells were pre-treated with Cyhex and uptake of 125 I-labelled apo-Lf and LfR abundance was assessed compared with untreated cells. ( A ) Apo-Lf uptake from cells significantly increased in the presence of Essay on A Most Writing cycloheximide (Lf+Cyhex) after 3 h compared with untreated cells (Lf). Motives For European! Data represent means±S.D. for two independent experiments ( n =3–4), and * P 0.05 compared with untreated cells, as analysed by Student's t test. ( B ) Representative immunoblot of on A Writing total protein extract isolated from cells pre-treated with Cyhex then incubated with apo-Lf for 3 and 7 h compared with untreated cells. The immunoblot demonstrates that Cyhex did not affect LfR abundance. ?-Actin was used as a loading control. CTs are specialized cells in the placenta that are initially responsible for invasion into the uterine endothelium, assuring optimal fetal development [10]. Our previous detection of motives exploration LfR along the what does nothing, maternal and fetal interface in the placenta implicates LfR in CT function [8,9,27].

The aim of the present study was to explore the function of for european exploration Lf in the placenta as mediated via LfRs using a CT cell model. While primarily regarded as a component of milk [2], Lf is also found in decidua (9–95 ?g/g), amniotic fluid (2–37 ?g/g) and chorion (2–26 ?g/g) [28], and Essay NOT Support the Death, is secreted directly from uterine epithelia [29,30]. This suggests that Lf secreted from the uterine endothelium may regulate CT function in a paracrine manner. Results from this study documented an enhancing effect of apo- but not holo-Lf on MMP-2 activity in CTs, suggesting a regulatory role for apo-Lf in exploration CT invasivity as this process is dependent upon the secretion and activation of MMPs. There is Bold and Brazen to Pursue Writing, precedence for motives exploration, effects of what is zone of proximal Lf on MMP regulation as over-expression of Lf activates MMP-1 expression through binding to AP-1 promoter elements and through stimulation of the p38 MAPK (mitogen activated protein kinase)-dependant pathway in motives for european mouse fibroblasts and human COS-1 cells [17]. We speculate that apo-Lf may similarly regulate the transcription of MMP-2 via AP-1 promoter elements which are present in Most Dream the MMP-2 gene [31]. In support of the for european, concept of Lf acting as a transcription factor, a nuclear localization signal within the N-terminal region of the and shiffrin memory model, Lf molecule (amino acids 1–5) has been previously identified [32], and Lf binds to motives for european, DNA [33,34]. Is Zone Of Proximal Development! Alternatively, the induction of MMP-2 via apo-Lf could be through the p38 MAPK pathway as p38 activation is required for the up-regulation of MMP-2 protein expression [31]. The ability of Lf to motives for european exploration, activate p38 MAPK has been previously shown in other cell types [16,35], providing support for a direct role of apo-Lf on CT invasivity.

The effect of apo- but not holo-Lf on MMP-2 activity probably reflects that distinct functions are associated with either apo- or holo-Lf. Introduction! In contrast to reports in rat hepatocytes [36], CTs bound and internalized both apo- and holo-Lf. However, in CTs, apo-Lf was internalized more avidly than holo-Lf, suggesting preferential uptake mechanisms specific to apo-Lf which may reflect the requirement of this molecule for for european exploration, CT-specific functions such as invasivity, while precise Lf-dependant functions in of poetry hepatocytes remain unknown. Gene attenuation of LfR conclusively determined that LfR primarily facilitates apo-Lf uptake in CTs. Recently, distinct Arg-Gly-Asp (RGD) binding motifs were found within the Lf molecule [37], which have been postulated to determine ligand binding and motives exploration, receptor interactions.

It is possible that iron-saturation, which alters Lf conformation, may result in LfR inaccessibility to these binding motifs. Memory! While this is not to imply that holo-Lf binding and motives for european, uptake does not elicit cellular events, it does not appear that these effects are mediated via LfR internalization in CTs. Possibly, holo-Lf internalization is not a prerequisite to how to, stimulate cellular events at exploration the plasma membrane as suggested by other investigators [15,35]. As apo-Lf was preferentially taken up by LfR, we sought to characterize the placental LfR more extensively. Does Much Nothing Mean! It has previously been reported that intestinal LfR is for european exploration, a GPI-anchored protein [3]. Contrary to this observation, we determined that, in CTs, the LfR receptor is is zone of proximal, not a GPI-anchored protein and is in motives exploration fact internalized via clathrin-mediated endocytosis following ligand binding. Our observations in trophoblasts are consistent with previous reports in other cell types such as hepatocytes, where inhibition of clathrin-mediated endocytosis by hypertonic medium [38] significantly reduced Lf internalization.

We speculate that differences in element Lf uptake between intestinal and CT LfR may reflect differences in motives for european exploration cellular function, as CTs are a semi-pleuripotent and on Christians the Death Penalty, invasive cell type, whereas enterocytes are terminally differentiated polarized cells involved in vectorial nutrient transport. Our determination that LfR is internalized via a clathrin-dependent mechanism may provide insight into LfR structure, as receptors utilizing clathrin-mediated endocytosis require a cytoplasmic, tyrosine-based sorting signal for interaction with adaptor proteins, such as AP2 [39]. To identify potential AP2-interacting motifs within the LfR sequence we used a proteomics server (www.elm.eu.org) and indeed two tyrosine-sorting motifs were identified at amino acids 120–123 and 129–142. This further suggests that a portion of LfR protein does reside intracellularly and that one or more of these motifs may functionally interact with AP2 to facilitate LfR internalization. LfRs have been identified in exploration various cell-types and reports have determined a molecular mass of ?110 kDa [8]. Recently, Tsuji et al. [40] examined the structure of human LfR in greater detail and determined that it is an 105.5 kDa oligomeric protein made up of introduction for assignment three 35.5 kDa subunits, similar to motives, what we observed in trophoblasts. Interestingly, while we detected both the what does, monomeric (35 kDa) and trimeric (105 kDa) forms of LfR under reducing conditions, the motives for european, abundance of the element, trimer was always greater that the monomer.

For some reason, we were unable to extensively reduce the trimeric form to monomers even when using dithiothreitol and iodoacetic acid (V. Lopez, unpublished observations). Interestingly, we did not detect the dimeric form of LfR under reducing conditions, which is similar to the findings of Tsuji et al. [40]. Exploration! We further demonstrated that apo-Lf binds to both dimeric and trimeric LfR multimers, which agrees with previously published reports demonstrating that human LfR forms a homo-trimer [7,40] as a result of subunit oligomerization [9]. Element! We speculate that the trimeric form is an unusually stabilized form, mediated by several intracellular disulfide bonds and possibly also hydrophobic interactions, whereas the dimeric form is motives for european, less stable and can be reduced to does nothing mean, the monomeric form. In contrast, a 45 kDa calcium-dependent LfR has been reported in rat hepatocytes [41]; however, it is unknown how this LfR relates specifically to Lf function as rats do not express Lf. It is well established that cell signalling and cellular homoeostasis are altered by increasing or decreasing cell surface receptor abundance [42]. As LfR abundance at the plasma membrane did not require de novo synthesis, our data suggest that recycling of LfR to and from the plasma membrane occurs. Endocytic recycling requires internalization of cell surface receptors and for european exploration, their ligands within clathrin coated pits [43], with the interaction between transferrin and TfR being the classic example of this process [44]. Receptor recycling is one strategy by which cells maintain a mobilizable receptor pool to quickly traffic to the cell surface in and shiffrin model response to physiological stimuli, to allow cells to redistribute cell-surface molecules to for european exploration, specific cell locations or compartments undergoing a physiological activity [45].

We speculate that Lf may either stimulate the on Christians Should, recruitment of LfR to motives, the plasma membrane, or that perhaps Lf binding to the receptor results in transient accumulation of LfR prior to internalization via clathrin-mediated mechanisms. Write Introduction! We propose that CTs may require Lf/LfR internalization to rapidly facilitate implantation, allowing for optimal embryo-fetal development. Further studies are needed to motives for european exploration, address this issue. Collectively, the much ado about nothing, results from this study demonstrated a specific role for apo-Lf but not holo-Lf in regulating MMP-2 activity and thus potentially endothelial invasion in CTs. Importantly, we have determined that apo- but not holo-Lf is internalized via LfR, which occurs through a clathrin-dependent pathway in CTs suggesting a mechanistic and functional difference between apo- and motives, holo-Lf. These results may help to explain the pleuripotent and cell-specific functions which have been associated with Lf.

The authors would like to express their gratitude to Dr. Lisa L. Gough for write introduction, critical reading of the motives for european exploration, manuscript. This work was supported by of proximal development NIH grant number HD-043240. Abbreviations: ALP, alkaline phosphatase; apo-Lf, iron free lactoferrin; CT, cytotrophoblast; Cyhex, cycloheximide; EEA, early endosomal antigen; GPI, glycosylphosphatidylinositol; holo-Lf, iron saturated lactoferrin; LAMP, lysosome-associated membrane protein; Lf, lactoferrin; LfR, Lf receptor; M6PR, mannose 6-phosphate receptor; MAPK, mitogen activated protein kinase; MMP, matrix metalloproteinase; Pi-PLC, phosphatidylinositol-specific phospholipase C; siRNA, small interfering RNA; SFM, serum-free medium; ST, syncytiotrophoblast; TfR, transferrin receptor.

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2017 MBA Essay Questions: UCLA Anderson. Motives! The Anderson essay guide is here to help you out with everything about your MBA app to UCLA! Thankfully, UCLA has fixed the awkward mission-focused attempt at what is zone branding within their essay question, and simplified it to what really matters: the goals. Motives! They also have a new short-answer question that lets you bring in another dimension. Describe your short-term and on Christians the Death, long-term career goals. How can the exploration UCLA Anderson experience add value to your professional development? (500 words) Describe how you would contribute to the UCLA Anderson community. (250 words) For the what nothing short-answer question especially, it wouldn#8217;t hurt to check out this video that they released in a prior session, so that you get a sense for motives for european exploration what they mean by their culture and how they value those who #8220;share success, think fearlessly and drive change#8221;: You can also refer to past essays and and shiffrin model, other discussion towards the bottom of this page.

Anderson is motives exploration one of the most reapplicant-friendly schools out there, and this is reflected by their very generous 750-word essay for those who are trying again: Reapplicant Essay Question: Please describe your career progress since you last applied and write, ways in motives exploration which you have enhanced your candidacy. Element! Include updates on short-term and long-term career goals, as well as your continued interest in UCLA Anderson. There#8217;s also a standard optional essay (250 words) so plenty of opportunity to exploration, talk to the adcom about element, things that matter in your app! We have updated our UCLA Anderson essay guide for 2017 to reflect these changes. Average GMAT for the Class of 2018 decreased from 718 to 716, and the upper end of the motives 80% range of scores ticked down from 680 to mean, 760, to for european exploration, 680 to 750.

We don#8217;t know about app volumes at this school but it appears that either they#8217;ve been getting fewer candidates applying #8212; which is opposite to every other program #8212; and/or they had trouble getting them to convert that year. Rd 1: October 6, 2017 one of the later ones, which means you#8217;ll get to take your breath after a crazy run in September with other schools#8217; deadlines hitting first. It also means that (hopefully) you#8217;ll be able to write introduction, do a better job on motives for european exploration your UCLA app because you#8217;ll be well experienced by of proximal development, then! Interview invitations often begin coming out quite quickly after the exploration deadline, and they continue throughout the whole cycle till December. There#8217;s nothing to is zone of proximal development, be interpreted from whether you receive one earlier or later in the sequence. Rd 2: January 5, 2018 another #8220;ouch#8221; Round 2 deadline (sigh nobody listens to the #8216;Snark) Two recommendations, as is common for motives exploration most schools. On Christians Should! One of them should be from your current boss and the second one someone else in a position of seniority. Three standardized questions for them to answer and one optional (See their questions here ) Lots of exploration resources for choosing your recommenders available here on the blahg; if you want more help on who to choose, our Letters of Recommendation App Accelerator walks through the specifics and and shiffrin model, lets you submit your recommenders#8217; strategy for feedback from EssaySnark. We don#8217;t have many Anderson essays reviewed on the blahg, however they look for the same strong career goals as Columbia, Ross, and motives, other schools do, so try these out for some guidance: For Reference: Anderson#8217;s Past-Season Questions. Included in case anyone wants to see what UCLA asked before. Click to view 2016 questions.

2016 Essays EssaySnark#8217;s Analysis. Here#8217;s what we said when last year#8217;s questions came out#8230; remember this analysis is from *last year*. Ado About Nothing! They#8217;ve kept the motives exploration question from 2015, which is: We believe that the best results are achieved when you share success, think fearlessly and what does much mean, drive change. With this in mind, what are your goals at UCLA Anderson and in your short-term and long-term career? Here#8217;s the video that they produced when they first came out with this particular essay: [end discussion of 2016 questions.] 2015 Essays EssaySnark#8217;s Analysis. UCLA thankfully improved their application this year: The difficulties in phrasing of the essay question that they introduced last year have been fixed, and for european, they#8217;ve also reverted to two recommendations, from atkinson and shiffrin, just one that they had required. These are good news for motives all you BSers.

Details below, and our UCLA MBA essay guide is up to date with these 2015 changes. Element Of Poetry! Cool! With this question, and the change in for european their Round 1 schedule, EssaySnark is pleased to see UCLA Anderson, like other schools this season, redeeming themselves from past mistakes and applicant-unfriendly policies. UCLA joins the trend of improving apps! Like MIT, Anderson simplified their one essay question it#8217;s still a little unwieldy but it#8217;s much much better than they had before. Here it is for the Class of 2018: We believe that the best results are achieved when you share success, think fearlessly and drive change. With this in mind, what are your goals at UCLA Anderson and in your short-term and long-term career? (750 words) They#8217;ve posted this video as a way to convey the what is zone of proximal #8216;culture#8217; themes those phrases #8220;share success, think fearlessly and drive change#8221; are all part of their school principles: They also pulled in their Round 1 deadline, to October 6th. That#8217;s still one of the later first-round deadlines for the top schools but it#8217;s now three weeks earlier than last year (it was way out on October 22nd then). This standardization actually may not help you all that much, since you#8217;ll likely be feeling pretty burned out by essay writing in the first week of October, having already managed submissions for about a month. Motives Exploration! The advantage is atkinson that you#8217;ll be in the swing of things with your essay writing projects and you will have plenty of experience writing essays for other schools by that time but the motives exploration temptation may be very great to what of proximal development, re-use one of for european those other essays for Anderson.

And that would be a mistake. When they had a three-weeks-later deadline then at least people could take a break and regroup after the flurry of craziness in September. So not sure this new standardized date is element of poetry that helpful. What will help is hopefully! that Anderson will begin issuing interview invitations much earlier now. Motives! That#8217;s to everyone#8217;s benefit. Much Nothing Mean! The 2015 UCLA Anderson application guide will walk you through what#8217;s important in your pitch including some critical policies that are rather unusual at UCLA compared to other schools. Be equipped for success with this detailed guide for motives for european UCLA! 7/29/15 update: YES! ANDERSON SWITCHED BACK TO TWO RECOMMENDATIONS AGAIN! We had hoped that they would do this, and they have. Of Poetry! (Last year it was only one rec which really put everyone at a disadvantage).

One of your recommendations should be from your current boss and motives exploration, the second one someone in on A Dream to Pursue a position of seniority. Three questions for that person to answer (review them here ) [end discussion of 2015 questions.] 2014 Essays EssaySnark#8217;s Analysis. 8/1/14 The UCLA essay prompt has been updated; it#8217;s still just one question about goals, but it#8217;s been expanded to conform with the trend of so many other schools talking about for european exploration, #8220;mission#8221; and #8220;principles#8221;. You shouldn#8217;t be using the optional essay for UCLA any more than you should for Essay Bold Writing most other schools (in other words, don#8217;t submit it unless you need it for something specific to motives, explain about a weakness in your profile). 2014 UCLA full-time MBA question Class of 2017. UCLA Anderson is element of poetry distinguished by three defining principles: Share Success, Think Fearlessly, Drive Change. Motives Exploration! What principles have defined your life and pre-MBA career? How do you believe that UCLA Anderson#8217;s principles, and the environment they create, will help you attain your post-MBA career goals? (750 words maximum) There#8217;s also a reapplicant essay, and an optional essay of up to on Christians Should NOT Support the Death Penalty, 250 words (remember that should be used only to explain stuff, not just to talk about motives, new things that you want to talk about). And, UCLA has mostly standardized with other schools asking just two questions of of poetry recommenders however just one recommendation is required. That#8217;s gonna be tough.

This year#8217;s essay question sounds remarkably similar to Haas and their #8220;defining principles#8221;; hate to call Anderson a copycat but this is far from for european exploration, original. Instead of Kellogg#8217;s #8220;Think Bravely#8221; we now have #8220;Think Fearlessly.#8221; We had actually applauded Kellogg#8217;s decision to drop language around their brand tagline from their essays this year, and now here is Anderson falling into element of poetry that same trap or more precisely, forcing all of you BSers into it. Motives Exploration! At least #8220;Share Success#8221; is easy to understand, though on first blush it#8217;s pretty one-dimensional, too. These types of essays are NOT easy to how to write for assignment, write. You need to spend time ferreting out what in exploration heck the school means by these #8220;principles#8221; and then you need to come up with stories from your past that illustrate how you#8217;re someone who shares them and THEN you need to Essay on A Dream, communicate your plan for your future. All of that in 750 words.

Sheesh. Not doing anyone any favors, UCLA. For European Exploration! [End 2014 questions section.] What are your short-term and long-term career goals, and how will an MBA from UCLA Anderson specifically help you achieve these goals? (750 words) The beauty with UCLA is they give you enough room to Essay Most to Pursue Writing, actually write something. This July 2013 post from Anderson Admissions explains their changes. [end discussion of 2013 questions.] What is your proudest achievement outside the workplace, and how has it impacted you? What are your short-term and motives exploration, long-term career goals, and how will an MBA from does, UCLA Anderson specifically help you achieve these goals? [end discussion of motives 2012 questions.] The UCLA MBA essay guide has been updated for 2017 to help you out with your essay and for assignment, short answer! 2017 MBA Application Strategy Guides.

The 2017 Darden MBA Application Guide - rewritten almost from motives, scratch to help you with the 2017 app! The 2017 Michigan Ross Essay Guide - totally new to help you with the nine short-answer options and your career goals! The 2017 MIT Essay Guide with brand-new material on what much the cover letter and the 'introduce yourself' video. The 2017 Duke Essay Guide - covers the 25 Random Things essay and all the rest too! The 2017 Harvard MBA Application Guide - refreshed with new details and strategies for your Class of 2020 app. Brave Supplicants' latest reviews on The 'Snark. I think that the reviewer raised a lot of good points, but a) three pages of feedback on a 250 word . Motives Exploration! As a re-applicant, I knew I needed considerable improvement to my pitch in order to maximize my . Thanks so much for the additional feedback ES.

I really appreciate it. I just submitted my HBS app, . What were we snarking about at this time in past years? 2016 : ($) If you get fired, do you need to tell the adcom? 2016 : Good luck for HBS applicants! 2016 : No, you don't have to how to for assignment, actually do the thing you say you will do in the career goals essay. 2014 : Did you just discover EssaySnark? 2014 : The disturbing trend of decreasing transparency 2013 : More on visiting schools 2013 : ($) Being a thought leader is not a career goal. 2012 : ($) Duke's main essay: What do you tell your family, friends, and colleagues? 2012 : About Deadlines. And Momentum. 2011 : Battle of the Blogs!

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Burn , Campfire , Camping 1249 Words | 3 Pages. ? A Good Camping Trip Why do so many people like going like going on a camping trip ? What makes . a camping trip that people call it good? In English, students use 5Ws (what, when, where, why, and who) and 1H (how) to make their essays look good. The same is true on exploration, a camping trip . There are also three Ws that can make a good camping trip which are when, where, and who. In order to have a good camping trip , when to go camping is essential. In other words, people need to choose a time it is suitable.

Camper , Campfire , Camping 445 Words | 2 Pages. TRIP Essay Conflict Resolution Who Do We Hire? Participants: John, Jim (the Director), Laura, Karl, Keith and . Celeste Setting: Mental health center Situation: An opening for a full-time therapist has been created by one of the and shiffrin memory, staff therapists quitting. Questions 1. Name at least one topic (T) issue, one relational (R) issue, one identity (I) issue, and one process (P) issue of this conflict. For European Exploration. T Issue = The most obvious topic issue during this conflict is the need to element, fill the position.

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It does not mean that it is appropriate to. Backpacking , Camper , Campfire 823 Words | 3 Pages. ? ENG111-001 Compare/Contrast Essay Final Draft 25, June 2012 Camping or . Resort I have always wondered what would be the difference between camping at a state park and a resort vacation. I looked into two different vacations that a family might enjoy, comparing the motives for european exploration, difference between what it would take to prepare for, the prices for is zone of proximal development, a week and exploration what is how to for assignment, offered with both. Primitive camping consists of a tent, fire ring and a picnic table. Amusement park , Florida , Magic Kingdom 1029 Words | 5 Pages. Personal essay on trip to Florida. Keightley Bertram. For European Exploration. I felt so lucky to be included in such a trip , adopted for element of poetry, a week by motives for european Keightley's family to atkinson and shiffrin, celebrate her birthday in style. . Exploration. Such an awesome party it was going to for assignment, be, with an unlimited amount of space, people, and lack of restrictions. This was definitely going to be a trip to be remembered.

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Air mattress , Campfire , Camping 864 Words | 2 Pages. that sort. Now, Dylan and I liked video games and movies, but this summer we wanted to motives, do something exiting. As the first day of school was getting near, . a senior at my school named Dave said to me, Hey, you and Dylan want to come along on a camping trip with some of my friends. ? I immediately shouted out, Yes, we sure would! ? I knew this was it, so I called Dylan right away and said, Guess where we are going this weekend? ? and what of proximal he said, where? ? By the sound of his voice, I could tell that. 2006 albums , Backpacking , Camping 446 Words | 2 Pages. Camping Brings Families Together I would like to focus on the camping experience and how that particular . recreation and motives leisure allows us to break away from work, tasks, household chores, and most modern conveniences, to encounter a very different kind of activity and play. One that is far removed from television, computers, MP3’s, and what does much texting on cell phones; this would be the simple process of camping . I propose to convince my audience that camping is a recreation. Backpacking , Campfire , Camping 924 Words | 6 Pages.

THE TRIP TO CUBA THAT ENDED IN LUNETA by for european exploration VW Bro. Bernardino L. Saplaco, Jr. Past Grand Pursuivant I simply want, in this article, to . recreate important events that culminated in Dr. NOT Support Penalty. Jose Rizal's execution on Bagumbayan field (now the Luneta), the motives exploration, centennial anniversary of which we commemorated on December 30, last year (1996); to pose at certain points, perhaps for polemical reasons, some pertinent questions which, to how to write introduction, me, still clamor for satisfying answers; to reiterate a couple of famous tributes. Capital punishment , Jose Rizal , Josephine Bracken 2073 Words | 5 Pages. How to Have a Sucesful Camping Trip. have a successful camping trip Are you bored to be at home on holidays?

Do you want to travel but you do not have enough . money?. Well, the best option to this horrible problem is motives exploration, taking a camping trip . A camping trip can be wonderful for of proximal, families and friends to spend time together in a relaxing setting at a relatively low cost. For European. Now, let me share with you the following three steps, so that you can have a pleasant camping trip . The first step to write, have a successful camping trip is choosing the perfect. A Perfect Place , Backpacking , Camping 537 Words | 2 Pages. Narrative Essay: Once More to the Lake. February 11th, 2010 Once more to the Lake is an essay written by E.B. in motives for european exploration, which the author tries to establish the links of his present life . with his past experiences when he was a little boy. The essay starts as a father and son go to Most and Brazen to Pursue Writing, the lake, which was a favorite place for camping and fishing of the motives exploration, father when he was a child. The father is full of expectations as the lake symbolizes his youth ages and the most careless period of his life. Of Proximal. So the decision to go fishing again on this lake may be. Change , Family , Son 1072 Words | 3 Pages.

October 7, 2012 How to motives for european, prepare to go Camping Camping gives an does much nothing individual an opportunity to be outdoors and motives for european exploration enjoy nature. . People either love or hate to go camping . I grew up camping every weekend during the summer and loved every minute of introduction, it. Camping gives you an opportunity to see places you have never seen before. Experience new things you would not be able to at your own home or in exploration, a city. There are a few things that you should do to prepare for camping : plan on where to go, plan how long you. Backpacking , Campfire , Camping 1018 Words | 3 Pages. destination and where they stay on the way.

Tent camping and nothing mean staying hotel are two of the motives for european exploration, most common ways of vacationing. Some people prefer . camping over element of poetry staying at a hotel because it is a good option for motives, a limited budget, it is adventurous, and campsites are surrounded by the great outdoors. Camping is and shiffrin model, a good option for a limited budget. Cost is often a key consideration. Motives For European Exploration. Camping is what development, less expensive than staying at hotel. The major cost of camping is the motives exploration, equipment such as tents, sleeping bag, camp. Camping , Hotel , Hotels 919 Words | 4 Pages.

Section 2357 Camping vs. Resort People like to go on vacations and spend time with ones they enjoy being around. Of Poetry. When traveling people focus . most on for european, where they would like to stay. Although camping in on Christians the Death, the woods and staying in a resort are the two most common ways to take a vacation, one should consider camping because it is less expensive, requires simple effort and its rejuvenating. There is a huge different in price range between camping in the woods to staying at motives a resort. When camping deep in the. Campfire , Camping , Campsite 869 Words | 4 Pages. Lake Tahoe, CA. this is a descriptive essay about my camping trip to lake tahoe, CA durring the fourth of july cellebration. lungs. The campground is what much mean, packed full of random campers from all across California and Nevada.

This is defiantly the busiest time of the year for motives for european, Lake Tahoe . Valley Camping Resort. Being so close to the big blue lake, there is no way I am going to miss the most incredible display of fire works in on A Most Bold and Brazen, all of California. Lake Tahoe Valley Camping Resort is located a few blocks down the motives for european, street of South Shore Lake Tahoe. When you enter through the resorts gateway, one feels as if he is visiting a small, remote. Arcade game , Camping , Lake Tahoe 1109 Words | 3 Pages. 1 Being in nature is the element of poetry, best place to be on earth. If you go or have been camping you know what this means. Motives For European Exploration. Being in the . Essay NOT Support The Death. outdoors opens your mind to motives, new things. For Assignment. Their's a lot that can be learned in nature, if people took the time for motives exploration, it. What Of Proximal Development. Nature and motives camping offer things that the Essay on A Most Bold to Pursue Writing, regular day can't.

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The very next morning I went to work and because. A Little Bit , A Little Bit Longer , Flight 1302 Words | 4 Pages. Jesse Karn English II Airbnb Travel Essay August 22, 2013 My Wonderful Trip to motives, Matariz Island Today was finally the Essay Should NOT Support the Death Penalty, big day, I . could not wait and that’s an understatement. Today was the day we left for motives exploration, our week long trip to a private island in what is zone, Grande, Brazil. Me and three of my closest friends have been planning this trip for over a year now! It’s about five here and we board our plane at six so you could say we are all very excited. After an hour of motives exploration, waiting it was finally time to board our plane. American films , Big Day Out , English-language films 614 Words | 2 Pages. How to Survive a Winter Camping Trip. How To Survive A Winter Camping Trip When you are planning a winter camping trip , you need to make . sure that you have all the necessary materials. If you don’t, then your trip may not go as smoothly as you think.

First of write introduction, all, I am going to tell you about what outerwear you need to bring. Stay warm, but control how warm you are. Pack clothing that can be easily layered and make sure each layer has good insulation and motives for european exploration will dry easily. This means that you should avoid packing cotton. Synthetic. Camping , Campsite , Need to know 674 Words | 2 Pages. Camping Technology Could something designed to make life easier end up ruining everything we have worked to how to write introduction, preserve?

Technology can . Motives. sometimes be quite controversial. In my major, I have found that it can be almost more of and shiffrin memory model, a frustration than a blessing. In most industries, technological advancements are viewed as a positive step towards efficiency and economic growth. This is not always the case, however. In the camping industry, for example, the constant “improvements” are often a source of. Camping , Frustration , Michigan 1375 Words | 4 Pages. all people said safety comes first.

SAMPLE ESSAY FROM FORM 2 ENGLISH MID-TERM EXAM 2011 Dear Surya, How are you getting on in . Penang? Did you manage to adapt yourself in the new environment there? I hope you can get along with all the teachers and students. Moreover, it will be as easy as ABC for motives exploration, you to make friends because you are a bubbly and introduction for assignment cheerful person in motives for european exploration, nature. Memory Model. Anyway, I am sorry for writing to you late as I went on a fun trip to an island with some friends for five days and. Andrew Lloyd Webber , Erik , Fishing 1372 Words | 5 Pages. ?My Camping Experience When I was in junior high school, I went on a school camping trip on motives, the 7th grade. I . loved the outdoors and had always wanted to try camping . So when the opportunity came my parents encouraged me to go even though I was a bit concerned about getting homesick since that was the and Brazen Writing, first time I was going to be away from my parents for any significant period of time or for 3 days. For European Exploration. We were going to camp in the camping area Jambore Cibubur.

It is my first experience to go to that. Campfire , Camping , Campsite 524 Words | 1 Pages. A Hunting Trip and Young Drivers: Two Essays. Sample Narrative Essay A Hunting Trip News of the what is zone development, tiger’s attack on a woman spread like wildfire. For European Exploration. This was not the does much, tiger’s . first appearance in the village. Before this attack, the villagers had lost a few goats and poultry but the disappearances remained a mystery as nobody could confirm that they were a tiger’s doing. Then the tiger struck. This time, the victim was a 32-year-old woman who was washing clothes by Tasik Bina.

The villagers at once organized a hunting party. Motives Exploration. Armed with machetes. Autobahn , Freeway , Road 766 Words | 3 Pages. Camping : Mountains and does nothing mean Desert A few times a year, I like to go camping . Sometimes I go to the mountains in Kern County, . California, whereas, other times I go to the desert in Brawley, California. Camping in the mountains, although similar in atmosphere, terrain and activities, has many more benefits than camping in the desert. There is motives exploration, nothing like taking a deep breath of cool, crisp mountain air, taking that narrow winding road that never seems to end until at Essay on Christians Should NOT Support Penalty last reaching the very spot that. Campfire , Camping , Campsite 944 Words | 3 Pages.

filled out motives for european exploration, my admission form and the lady on the Admiration told me that I have to take assessment test, after that I can enroll in classes. I was new in the . environment so I didn’t know what assessment test is. At that time my brother was on business trip so my mother told me not to element of poetry, bother him and try to figure out by myself. I was very sacred I didn’t know what I am going to do. I decided I am not going to motives, sit here and do nothing. What Much Ado About Mean. Next morning I went to college and asked the administration lady what. Academic term , College , Essay 1524 Words | 4 Pages. When someone says to their parents, “You guys want to pay for a trip to England for me, don’t you?” and they say yes, then a certain amount of . Motives For European Exploration. worry comes to that person immediately preceding the flight out ado about mean, of the country. Will I like England? Will I learn anything?

Will I enjoy all the motives for european exploration, places I saw on TV? Will the British hate me? Am I going to write for assignment, hate it and motives for european exploration blow several thousand euros (well, pounds when I exchange it) my parents spent on on A Most and Brazen Dream to Pursue Writing, me for a favor I was joking with them about and feel incredibly. Anxiety , Belvoir Castle , Sherlock Holmes 2076 Words | 5 Pages. An Analysis of E.B Whites, “Once More to the Lake” In E.B Whites essay , “Once More to the Lake” he reflects on his summer outing with his . Motives For European Exploration. son. Should NOT Support Penalty. Throughout the trip , memories of his childhood, long forgotten, resurface themselves as he experiences the motives exploration, same vacation with his own son. These memories create in him a feeling as if time has not changed and that he is reliving his old days. His father used to take him to the same camping spot as a boy. He was certain that there would be changes since then. Emotion , Feeling , Narrative 1092 Words | 3 Pages. of view European Lebanese School Extra Sheet Subject: English Grade: 10 Teacher: Rana Chehade Section: A Term: I Date: Name of student: . WRITING ESSAYS OF COMPARISON AND CONTRAST Essays of comparison and contrast discuss differences and does mean similarities between two ideas, subjects, or issues. ? Model essay 1: Gender Differences The battle between the sexes started with Adam and Eve, and will probably continue forever.

The opinion that men are superior to women has long. Brain , Difference , Female 980 Words | 6 Pages. THE WINNING ESSAY IDEA is happy to announce Aisa Ovshiyeva from Russia the motives exploration, winner of the IDEA Declaration of Interdependence . essay contest. Honorable mention also goes to Syed Hashim Zaidi, the ?rst runner up from how to write introduction for assignment Pakistan and Feshko Yliana the second runner up from motives for european exploration Ukraine who will receive IDEA publications. Aisa will receive a trip to the Interdependence Day Celebration in Rome, Italy on September 12, 2004. Idebate Magazine would like to congratulate Aisa and we invite our readers to what of proximal development, read. Africa , BBC World Service , Globalization 1182 Words | 4 Pages. Camping Trip Finally, the time was here. It was fall break, and I had nothing to do but sit around and motives for european exploration enjoy life.

This . area was covered with dirt, and no matter how hard I tried, I would get filthy. I couldn't wait to get home to take a nice shower. It was a nice place to Essay on Christians Should, sit around and clear my mind. In the for european, middle of September, my mother and I would go to what mean, the store to buy tons of food for hunting trip . At last, after being at the store for hours, we would be on our way to meet. Camping , Family , Hunting 1617 Words | 4 Pages. Essays are generally scholarly pieces of writing written from an author's personal point of view, but the definition is vague, overlapping with . those of an article, a pamphlet and a short story. Essays can consist of a number of elements, including: literary criticism, political manifestos, learned arguments, observations of daily life, recollections, and reflections of the author. Almost all modern essays are written in exploration, prose, but works in verse have been dubbed essays (e.g.

Alexander Pope's. Alexander Pope , Essay , Essays 1053 Words | 4 Pages. probably noticed, essay writing assignments can pop up in any class. An essay is a literary composition that expresses a . Atkinson And Shiffrin Model. certain idea, claim, or concept and backs it up with supporting statements. It will follow a logical pattern, to include an introductory paragraph (make the claim), a body (support), and a conclusion (summary of statements and support). English and literature teachers use them on a regular basis, but essays are required in many other types of classes. Essay exams are also a. Abstraction , Essay , Fiction 876 Words | 3 Pages. disquisition, monograph; More 2. For European Exploration. formal an attempt or effort. a misjudged essay synonyms: attempt, effort, endeavor, try, venture, . Write. trial, experiment, undertaking his first essay in telecommunications a trial design of a postage stamp yet to be accepted. Motives For European Exploration. verbformal verb: essay ; 3rd person present: essays ; past tense: essayed; past participle: essayed; gerund or present participle: essaying e?sa/ 1. attempt or try. essay a smile Origin late 15th century (as a verb in the sense ‘test the. Definition , Essay , Gerund 608 Words | 4 Pages. Semester 1, 2013 Assessment Task 2:Critical Essay IDEAS in MANAGEMENT Writing instructions and Essay on Christians Should NOT Support Penalty Marking Rubric This assessment task is . an ESSAY . Exploration. The RMIT College of Essay on A and Brazen to Pursue Writing, Business requires you to use a particular style of essay writing which involves both the way the essay is structured and the way that you acknowledge other people’s ideas used in your work.

The structuring of an essay is very clearly described in for european exploration, the RMIT Study and write for assignment Learning Centre Essay Writing Skills Online Tutorial available. Article , Citation , Critical thinking 807 Words | 3 Pages. care of, flights have to for european, be scheduled, and accommodations have to introduction for assignment, be arranged for when the flight arrives. Motives. Also, it is important that some entertaining . sites have been chosen ahead of time that would be nice to visit. Element. By doing this, it will make the trip much easier to manage and much more enjoyable. Vacationing in motives, a foreign country can be a great experience for is zone of proximal, family and friends, but it is important that travel arrangements, accommodations, and entertainment options are researched thoroughly before. European Union , Hotel , Japan 865 Words | 3 Pages. ? Essay Instructions You will write 4 essays (double spaced, 12-point Times New Roman font). Motives. The first essay must . be 1,000–1,200 words, and the following essays must be 750–1,000 words each. Essay one corresponds to the essay one prompt as listed below. Essay two corresponds with the essay two prompt, etc. through all four essays . Each essay is a separate assignment.

In completing each essay , research must be conducted through 2–4 peer-reviewed, scholarly sources in addition to how to write for assignment, the Bible and the. Bible , Religious text 990 Words | 3 Pages. Bankers Adda How to motives for european, write Essay in SBI PO Exam? Dear readers, as you know that SBI PO 2014 Paper will also contain a Descriptive Test of . And Shiffrin Model. 50 marks (1 hour duration), which will consist of motives, English Language Comprehension, Short Precis, Letter Writing Essay ). So, here we are presenting you How to write Essay ? and few points to remember while writing an essay in the exam, which will be important for upcoming SBI PO exam. How to write an essay ? 1. Analyze the prompt. Note exactly what.

Essay , Jawaharlal Nehru , Linguistics 812 Words | 4 Pages. INTRODUCTION Going on a Educational trip means more than simply leaving the school grounds. Educational trips should always . have a major educational element, but the impact of Educational trips can extend much further. The importance of Educational trips includes giving students the chance to build closer bonds with their classmates, experience new environments and enjoy a day away from the and shiffrin memory, classroom When students and teachers are together outside the classroom, new educational environments. Education , Educational psychology , History of education 841 Words | 3 Pages. ?Ruiz 1 Liliana Ruiz Ms. Bancroft English 1 / Period 1 10 October 2013 Response to Literature Essay “Can’t stop,’ he thought ‘Mark’s in . big trouble.

Gotta keep going.’” In the short story, The Hiking Trip , Jeff has to travel through a canyon alone to seek help for his brother, Mark. The author reveals Jeff’s personal characteristics through his actions, revealing him to be caring, determined, and brave. Even though Jeff was really scared; he proved that he was very caring. For European. In the beginning. English-language films , Family , Father 506 Words | 2 Pages.

Ashley Hicks Hendren ENG 101 Essay 1 A Trip to Italy Vacation and travel is a phenomenon which strikes a chord with nearly . every human being on of poetry, the planet. While true, there are many without the means to motives exploration, engage in such activity, there is little doubt that the atkinson and shiffrin memory model, primal urge to escape one's everyday surroundings strikes all but the most reclusive of people. There are many different locales to motives for european exploration, which one may escape, as well as a large variety of reasons for traveling. What Is Zone Of Proximal. Be it sandy beaches, or. Colosseum , Culture of Italy , History of Italy 1154 Words | 3 Pages. Rhiannon Romero Ellen Maresh The Essay January 23, 2010 Road Trip “Tiiiim! Is the motives, car all packed? Are you ready to go?” . I yelled, as I ran up and down the stairs trying to on A and Brazen to Pursue, get everything together. He answers, but I only half hear him. Motives For European Exploration. I check and on Christians NOT Support double check the list in my head, “Food, check; blankets, check; pillows, check; games, check; camera, check; suitcases, check; gas, check; music CD’s, check; family, check…Lets do this!” I went downstairs to the playroom and the kids are watching.

Beach , Britney Spears , Dune 1058 Words | 3 Pages. their motivation was will determine if their act was moral or not. Motives Exploration. By betraying his own kin Momutu decided to give up his humanity, while on the other . Element Of Poetry. hand, Amoo gave up his own freedom to protect his family. Testing with success series The Essay Exam Organization and neatness have merit Before writing out the exam: Write down their key words, listings, etc, as they are fresh in your mind. Otherwise these ideas may be blocked (or be unavailable) when the time comes to write. African slave trade , Answer , Atlantic slave trade 857 Words | 4 Pages. BM 6105 Assignment BM 6105 Managing Change Assignment (3000 words) Essay Due on Monday 14th of January 2013 You are required to motives for european, write an . On Christians NOT Support The Death. essay supported with reference to the academic literature that answers the following question: • You have recently been appointed to your first management post following graduation. You are keenly aware that as part of your management role you will be responsible for motives for european, managing change and anticipate drawing on your BM 6105 studies to help you achieve success. Essay , Management , Organization 690 Words | 3 Pages. Argumentative Essay Social responsibility is an ideal topic for what, debate; there have been mixed results for companies and individuals who have . pursued social responsibility. Motives For European Exploration. There is also the question of whether social responsibility should be motivated by of poetry a perceived benefit.This type of essay is based on for european, philosophical theories on the necessity of social responsibility backed up with facts about previous social responsibility efforts.

For example, an essay could be about what does much ado about how giving support to disaster. Essay , Essays , Qualitative research 555 Words | 3 Pages. create flashcards for free at Cram.com Sign In | Sign Up StudyMode - Premium and Free Essays , Term Papers Book Notes Essays . For European. Book Notes AP Notes Citation Generator More Code Napoleon and “Declaration of the Rights of Man” Comparison By wis2cool, april. 2013 | 5 Pages (1064 Words) | 1 Views | 4.5 12345 (1) | Report | This is a Premium essay for upgraded members Sign Up to access full essay DID YOU LIKE THIS? TELL YOUR FRIENDS. Send Code Napoleon and atkinson model “Declaration. Age of motives for european exploration, Enlightenment , Declaration of the Rights of Man and Essay Most and Brazen Writing of the Citizen , French Revolution 632 Words | 4 Pages. ELEMENTS OF AN ESSAY Preliminary Remarks Following are some suggestions to help you write an acceptable academic- level essay . . For European Exploration. This is not the only way to how to introduction, organize and develop an essay . It is, however, a tried and true system and will likely be what your TCC instructors require of you. Audience and Purpose Before beginning, you should consider both your audience and purpose. For, before you can know how to approach the subject, you must determine whom you will be addressing, how much they already.

2005 albums , Essay , Five paragraph essay 1430 Words | 5 Pages. finished. If you choose to go to the toilet off a tree, make sure it is stable and make sure you are not going to fall. When digging a hole, make sure it big . Motives Exploration. enough and what ado about nothing also make sure it is not dug to skinny, or too wide. Water- While hiking, or even camping ; always carry a camel pack full of water. For European Exploration. If you don't own a camel pack, bring plenty of water. Preferably around 2 or 3 litres.

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How to Guide to Racing Resume / Sponsorship Proposal. So you want to motives exploration, race. Welcome to the club. Whether you are trying to work this out with family support, or on your own, unless you are fabulously wealthy, you have already realized that sponsorship is a must for nearly every racer on the track. In your pre-racing days you had to pay for parts to make your ride go as fast as your buddy's, or to make it easier on your bod while having fun.

As a racer, whether you are a first year amateur or a factory pro, you will have to obtain the what development, performance modifications and replace product when you mess it up, on or off the track. In fact I know of quite a few times that perfectly good brand new parts have been destroyed in motives the process of installing them (an expensive education and a lesson in why we should read the directions that come with our new toys). The point being that your little obsession/habit of for assignment racing/riding is exploration going to be a costly adventure, but also the time of how to write introduction for assignment your life. Anyone that has been to a race track has noticed the stickers on the bikes and haulers. For European Exploration? These are sometimes put there because they came free in the shipment of nerfs, handlebars or in a sticker pack the does much nothing, rider got at the local Honda shop. Motives For European? If you are a sponsored rider, then the branding/stickers on your quad and hauler establish you as a part of the company that you represent. Your actions while wearing the stickers of your sponsor reflect on the sponsor as well as yourself.

Sponsors consider that when choosing riders. Next question, How do I get a bunch of those stickers without paying for them or the parts they come with? Answer: You do your darndest to Essay Bold and Brazen to Pursue Writing, earn sponsorship. Simple. Right? Not really. When I first got into this arena I was in the same shoes you are in motives right now (unless you have a marketing degree- in of proximal development which case disregard the rest of this article).

My background had nothing to do with how to write a resume for motives, a racer. Sure I could find examples from books at the library or bookstore on resumes, but none for a professional or semi-professional athlete, much less a racer. What? So I had to determine what to motives for european exploration, do to mix the Most Dream Writing, style of resume that I would submit if was looking for motives for european exploration, a position with a secretarial pool or a teaching position and that of a professional athlete forming a `Racer's Resume'. If you are still reading this right now, you are probably one of the sponsor hungry population who are running out of Essay on Christians NOT Support Penalty time to get noticed and be considered for this season's budget from motives exploration most companies. How it works: Nearly every company has a `Sponsorship Budget' in their program. Each company has a person or team of persons responsible for distributing the monies/products or a combination of both to deserving athletes. This person or team of persons is called `Rider Support'. Nearly every company that offers sponsorship has levels of sponsorship. Common levels are A, B C level support, Pro and Factory.

Support can range from a discount on certain products to free products, contingency (this would be money paid for your wins at certain events) all the what much ado about, way to the Holy Grail of the racer: Factory Support. Now let's get down to how to motives exploration, keep your resume on the top of the pile and out of the trash can. Aside from the basics like your name, street address, city, state, zip code, home and of poetry, cell phone numbers, you should also include your email. A common mistake, believe it or not, is to leave out contact information. Jack Bednar, from Lonestar Racing, has had to dig through the motives for european, trash pile before to find the right envelope that a resume came in to find out how to get in touch with the rider. Also necessary in your resume is a short bio of you and your riding history. Don't go into great detail of how to write for assignment every second, third or less finishes you have had, and exploration, don't include reasons why you didn't podium at specific races. Until you are sponsored, no one really cares why you didn't finish a race except your parents. Photos are a must. You need to include a head-shot of yourself, one of how to write introduction your bike and one action shot of you at the very least. References must be listed on your resume.

Make sure to include contact information on your references as well. Submit your resume to the right people, during the right time frame. Motives For European? What this means is make sure that you address your resume to the correct department within your target sponsor. Check on the target sponsor's website or call their operator to make sure you have the element of poetry, right address, right name and motives for european exploration, right time frame to submit your resume. OK, these are the element, rules. Sounds pretty easy so far. If this was all there is to it, you would not have read this far. This section is more the strategy than cheat codes. There aren't really `Cheat Codes' to a resume, but there are bonus points you can score. Let's review what we have done so far. We have a basic 4 page resume.

You have included all the `regular' stuff and are following all the rules. Now, let's get creative and find the for european exploration, way to keep your resume on the `keep' pile of your target sponsor's desk. First of all do not even consider handwriting your resume. Make sure you use a good word-processor and spell check it. Kevin Mummuah, from Lost Creek Cycle, says, Don't expect someone to do more for you, than the effort you put into your resume. I can tell how much work they put into it when I open them.

The ones that haven't spent the time are obvious. Your focus should be to maximize what you can offer your potential sponsor in Essay NOT Support the Death the form of endorsing their product and how to communicate that you can do this to the sponsor. If you are sending resumes out to companies that you really like, then it shouldn't be too hard to express your enthusiasm for the company and their product or to come up with ideas on motives how you would carry the message to potential customers on how awesome their products are. Is your brain full yet? Maybe this is where you should reconsider that job at the sawmill instead of racing. I know I have suggested something like this a couple of Should the Death Penalty times to my racer. Beginning with your contact information review what you have there. Format your information with your name in motives for european exploration bold print. This is important.

If you have included your email, and I am hoping you ARE including an email, you need to what is zone of proximal, keep it professional. Motives Exploration? You are asking companies to invest a serious amount of confidence in you as well as money in you to how to for assignment, support their products and motives for european, represent their company. If you have an write introduction for assignment email of toker420@hotmail.com or radicalrider69@yahoo.com (I don't know if these are real emails or not), it definitely gives a certain impression. Most companies have so much interest in their sponsorship programs that they can throw away any potential riders that even hint of unprofessionalism. Go ahead and motives exploration, keep your identity nickname for chat and private messages, but obtain a professional email with your name in it like dana@danacreechracing.com or john_smith@hotmail.com either of these would be acceptable and have no unprofessional image. A note on professionalism: if you include your cell phone number or private home number make sure that your recording is also mainstream. No radical music interludes, raps, or weird recordings. Keep it short and much nothing, sweet. People in rider support don't have all day to listen to motives for european exploration, your favorite tunes or you goofing off on your answering machines. Make the choice to go with something grown-up, since you are asking for what much ado about nothing mean, a grown-up gift.

Most of the motives for european, Rider Support people take about 30 seconds on each resume before deciding what pile to file it in. You need to get their attention to make them even slightly interested in you in write introduction for assignment the first 10 seconds so they will put you in the `keep' pile rather than the `round' file next to the desk (trash can). Start your resume with an eye catcher. Motives Exploration? What catches your eye? An action photo would be a good idea. OK, here comes the write, kicker: you actually need a decent action photo to put there. If you don't have some that have been professionally done, get some. Next make sure that the reader can tell it's you. Below are two examples.

They are both great `action' photos but the for european exploration, second one is defiantly more eye-catching. Also use good sizes but not to overwhelming and use good quality prints. Essay Most And Brazen To Pursue Writing? After you have your resume completed don't mess it all up by printing on your home computer with a low color cartridge. Motives For European? You can have them printed in color on a laser printer for introduction for assignment, under a buck a page at the local Kinkos. Make sure that you are the focus and that action is clear. As Kevin Mummuah said, Don't send me substandard photos with ant people in them.

Next you need to describe what your abilities and motives, qualifications are. Get rid of all the and shiffrin, descriptions of how cool you are and what people think about you. Do include action words describing what you can do. Be willing to back it up. And keep it short. Make sure to motives, include a short history of your wins.

Don't include every race you did or why you didn't podium or finish a particular race. Atkinson And Shiffrin? If you have a photo of your pit area at a race this would be a good place to motives for european, include it. I am thinking of a photo of your pit area, neat, no garbage, clean well maintained quad, your tools organized and what of proximal development, maybe you and your mechanic (or Dad, or your dog) standing next to your quad in full clean gear. Remember you are asking a company to motives, be identified with your professionalism. Do you really think they will be interested in being associated with someone who has no respect for their bike or surroundings? Include where you plan to the Death Penalty, race for the upcoming season. Motives For European? Just list the series that you plan to do in full. If you are going to do something like the full GNC National schedule then include it, otherwise say you will be attending selected National events. Be realistic on where you are going to race and choose one series to on Christians NOT Support the Death Penalty, focus on.

Spell check. Read it aloud to someone (that usually catches something that is worded weird). Check your formatting. Make sure you don't have lines that look. this in your resume. Do your homework.

Find out exactly who to send your resume to by calling the motives for european, company or looking up on their website for information on where and who to send your resume to of poetry, AND make sure that you are submitting your resume in motives the right time frame. For instance Shift's Rider Support head, Rob Salcedo, says that they ONLY accept resumes during October 1-31 period. NOT Support The Death Penalty? Any resumes that show up even one day early or one day late go straight into the garbage can. Shift gets over 300 resumes from amateur riders in the ATV discipline alone. Clancy Schmitt, from Hiper-Technology, says they get over 500 per year. For European Exploration? The competition is stiff for those sponsorships.

So make sure yours comes to the attention of the right person during the right time period. Spell out of poetry, how you plan on earning your sponsorship. Motives? Simply `promoting my sponsors to the best of what much nothing my ability and listing my sponsors on motives my sign-ups' is not enough. This is EXPECTED not a perk from you. Running the sponsor's stickers and what, saying `Thank-You' when interviewed are also expected. Whew, now that we have that out of the way, think on what YOU can do that only motives for european exploration, you can do. Be original.

Be clear on NOT Support the Death Penalty what your plans are. Spell out exactly what you would like your sponsor to do for you. Do not expect free stuff. More realistically you can expect to receive some kind of a discount on product if you are selected for exploration, sponsorship. Many companies offer their main products at a special rate for sponsored riders.

If for introduction for assignment, instance you are approaching LoneStar Racing, you can let them know what products you are planning on purchasing and exploration, when you would need to make a decision on purchasing. Fasst Company said they want to see at least six things that you can do for Essay Most Bold Dream to Pursue, them. Chris and Cole say, Whatever you want from this company, you need to be prepared to do double in return. References are important. Just like a `regular' resume, be prepared to motives for european, supply the names, addresses, and phone numbers of people that have knowledge of is zone your riding and for european, personality.

Your school counselor could write a quick letter of support (on school letterhead) as well as be a reference, a family member, the how to introduction for assignment, owner of the for european, local bike shop and other sponsors are great references. Other riders are not good references. If you can get someone who is mean nationally recognized to be a reference this is best. The ATV industry is quite small and many people know each other well enough to motives, just give a quick call. If you are under 18 years old, it is absolutely imperative that you include the fact that you have family support. What Much Nothing Mean? Even young adults will benefit from showing a strong family support system. Be prepared to show who you are, not just what you do. Motives For European Exploration? This is hard to accomplish in how to write for assignment a 4 page resume but can be done. Presentation.

Make sure that you have a cover page, your resume, history/bio page, and for european, a photo page. This is all standard stuff that most of the element of poetry, Rider Support personnel that I spoke with wanted to see. They also have some pet peeves of what is not cool to them. Rob Salcedo absolutely hates those slippery covers that you get for school reports. What happens is exploration you get a stack of them together and they all start sliding out, ending up on the floor, in the coffee etc.

So binders are a big no-no. A simple staple or cover that is not slippery is what is zone of proximal development good. Motives? Have I mentioned spell checking yet? Take pride in yourself and your request. What Much Nothing? Let your potential sponsor see who you are. Biggest mistakes include: non typed or non word processed resume; slippery folders; the quote, `will promote your company to the best of motives exploration my ability'; large files emailed to company; too short of a resume; too long of what a resume; and misspelled words. Every company that I spoke with accepted resumes in mail form. Motives For European Exploration? A few accepted emails, and what of proximal, fewer still accepted FAXed resumes. Nearly every company accepted resumes in motives for european October and November. A few accepted them in September and a few accepted them in December. After January 1 no one that I spoke with would look at a resume.

Each company that I spoke with had different levels of support. Scot Denton, from Denton Racing, said they have four levels of support. Kevin Mummuah from Lost Creek Racing works with a lot of riders at NOT Support the Death different levels of motives support. Lost Creek has their pros but also a large number of amateurs and element, pros who are not even on the roster for Lost Creek, who sometimes end up getting on track support from Kevin. Hiper Technology has three levels of motives for european exploration support. Basically most of the companies that I spoke with have different levels of increasing support the longer you have been with the atkinson and shiffrin, company or as your racing wins or popularity as an ATV personality increases. If you refer a customer to for european, your sponsor, ask the customer to tell your sponsor where they found out is zone development, about the product. This looks very good for you and your sponsor will remember this when it comes time to re-evaluate their budget the next year. Jack Bednar summed it up pretty well, Obviously we can't see them all ride, so we have to go on exploration how they present themselves to us through a resume. There are a lot of people using the Sponsorhouse system for generating a resume.

This is a great resource, and OK to use, for a starting point. The format of the Sponsorhouse system takes care of all the basics but the how to write introduction for assignment, individuality is lacking and many times it seems that the potential sponsor or overwhelmed Rider Support personnel are directed to a web-page to go over. Motives? Keeping in atkinson mind that the Rider Support people see so many resumes each year, do you really think they are going to be interested in sitting in front of a computer surfing your webpage? Sponsorhouse is a great addition to motives for european, a traditional resume that is sent out as a hard-copy to your target companies and can really help as the year goes on by keeping your hard-won sponsors up to date on what is happening with you. I would not rely on Sponsorhouse to do it all for you though. If you end up getting sponsorship, and I am sure you will if you follow the tips in this article, make sure that you keep contact with your sponsors. Even if it is of poetry a small sponsorship the first year, you need to start somewhere.

Cultivate your relationships and in a few years you should have a much better sponsorship, assuming you live up to what you say you will do for your sponsor. Johnny Jump, from KN Engineering, says he likes to see photos of the bike with his company's stickers on it. Motives Exploration? It would be a fatal mistake to apply for a second year including a photo without your sponsor's sticker visible. Keep in much nothing mind all the riders you have seen in person, on the track, movies, magazines and on the internet. They are all potentially competing with you for the sponsor budget. You have to sell yourself. I know this is kind of weird but basically the `you-ness' of you is motives for european exploration all that really separates you from any other rider who applies for sponsorship.

I know it feels odd but you need to bite the bullet and go for it. As an added bonus you can view Dana Creech's 2003 Resume online through his website. Have fun, ride safe and don't forget to call your Mom.